Sirna sequences targeting

PLGA, with a lactide-glycolide ratio of 50:50 and Mw of 7,000-17,000 (ester terminated), PEI (average Mw 25,000 Da), poly (vinyl alcohol) (PVA, 30-70 kDa) and Coumarin-6 were provided by Sigma Aldrich. DiR Iodide and DiO were obtained from Yeasen. PTX was purchased from J& K Chemical Technology. The Avanti mini extruder and polycarbonate porous membrane were obtained from Avanti Polar Lipids. Antibodies targeting Rb (4H1), p-AKT (D9E), AKT (40D4), E7 (F-7) and MDR1 (D-11) were bought from Cell Signaling Technology and Santa Cruz Biotechnology, respectively. AS1411 aptamer (Ap sequence: 5′‐SH‐C6‐TTGGTGGTGGTGGTTGTGGTGGTGGTGG‐3′, 28 bp) was synthetized by Sangon Bioengineering (Shanghai, China). siRNA sequences targeting E7 (sense 5′-GCGACUCAGAGGAAGAAAATT-3′, antisense 5′-UUUUCUUCCUCUGAGUCGCTT-3′) and Cy5-labeled siRNA-NC were synthetized by Shanghai GenePharma.
The human cervical cancer cell line HeLa was obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The cells were cultured according to the guidelines recommended by the ATCC.
Female BALB/c nude mice were obtained from Shanghai Jiao Tong University School of Medicine and raised in the SPF animal facility. All the animal experimental procedures were conducted with the approval of the Animal Experimentation Ethics Committee of Jiao Tong University.

PTGIS lentiviral overexpression were purchased from Hanbio Tech (Shanghai, China). SiRNA sequences targeting PTGIS were procured from GenePharma (Shanghai, China). Sequences of siRNA are listed in Supplementary Table 2. Lipofectamine 3000 (Invitrogen) was employed to effectuate the transfection of cells with the small interfering RNA (siRNA), as directed by the manufacturer’s stipulated guidelines.