Quantiglo human il 6 elisa

Manufactured by R&D Systems

Sourced in United States

The QuantiGlo Human IL-6 ELISA is a quantitative sandwich enzyme immunoassay designed for the measurement of human interleukin-6 (IL-6) levels in cell culture supernates, serum, and plasma.

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ELISAs (144 citations) Human IL-6 (49 citations) QuantiGlo (10 citations) QuantiGlo ELISA (5 citations) Human IL-6 QuantiGlo ELISA Kit (2 citations)

4 protocols using quantiglo human il 6 elisa

Quantifying Plasma IL-6 Levels

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Plasma concentrations of IL-6 before and after the exercise were quantified by using a QuantiGlo Human IL-6 ELISA (R&D Systems, Minneapolis, MN), following the standard manufacturer's guidelines. All samples were analyzed in duplicate, with assay sensitivity of 0.4 pg/mL, and assay detection range between 0.50 and 1500 pg/mL. Intra-assay coefficient of variation was calculated as 4.5%.

Coco M., Perciavalle V., Cavallari P., Bolzoni F., Graziano A.C, & Perciavalle V. (2017). Effects of age and sex on epigenetic modification induced by an acute physical exercise. Medicine, 96(44), e8325.

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ELISA Analysis of IL-6 in Plasma

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A QuantiGlo Human IL‐6 ELISA (R&D Systems, Minneapolis) was used in order to quantify pre‐ and post‐exercise plasma concentrations of IL‐6 as per the standard manufacturer's guidelines. Samples were assayed in duplicate, including positive controls and various dilutions of the IL‐6 standard which were used to plot a standard curve. Assay sensitivity was 0.35 pg·mL⁻¹, and the assay detection range was between 0.480 and 1500 pg·mL⁻¹. Intra‐assay coefficient of variation was calculated as 4.3%.

Horsburgh S., Todryk S., Toms C., Moran C.N, & Ansley L. (2015). Exercise‐conditioned plasma attenuates nuclear concentrations of DNA methyltransferase 3B in human peripheral blood mononuclear cells. Physiological Reports, 3(12), e12621.

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Quantifying IL-6 Secretion in HPAECs

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We used the Human IL-6 Quantiglo ELISA (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions to determine the concentration of IL-6 protein released from HPAECs cultured under various conditions.

McCord J.M., Hybertson B.M., Cota-Gomez A., Geraci K.P, & Gao B. (2020). Nrf2 Activator PB125® as a Potential Therapeutic Agent against COVID-19. Antioxidants, 9(6), 518.

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Quantifying IL-6 Secretion in HPAEC

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We used the Human IL-6 Quantiglo ELISA (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions to determine the concentration of IL-6 protein released from HPAEC cultured under various conditions.

McCord J.M., Hybertson B.M., Cota-Gomez A, & Gao B. (2020). Nrf2 Activator PB125® as a Potential Therapeutic Agent Against COVID-19. bioRxiv.

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