Dual esi q tof 6520

Melting points were determined in open capillaries with a digital melting point IA9100series apparatus (ThermoFisher Scientific). All reactions and purity of the synthesized compounds were monitored by TLC using silica gel 60 F254 aluminum plates (Merck). Visualization was accomplished by UV light. Column chromatography was performed using silica gel 60 (0.040–0.063 mm) (Merck). NMR spectra were recorded on a Bruker Ascend 400 spectrometer (400 MHz and 100 MHz for ¹H and ¹³C, respectively). ¹H NMR and ¹³C NMR were referenced to residual solvent peaks. High-resolution mass spectrometry (HRMS) analyses were carried out on a Dual-ESI Q-TOF 6520 (Agilent Technologies) mass spectrometer.
The starting compounds 1 [39 (link)], 2 [39 (link)] and 19 [40 (link)] were prepared following the reported methods. Synthetic details, characterization and analytical data as well as ¹H and ¹³C NMR spectra of all synthesized compounds are presented in the Supporting Information File 1.

All chemical reagents and solvents were obtained from commercial suppliers (Sigma-Aldrich (St. Louis, MO, USA), TCI-Europe (Zwijdrecht, Belgium) and Merck (Darmstadt, Germany)). The purity of the compounds was checked by TLC (silica gel 60 F254 aluminium plates (Merck, Darmstadt, Germany)) and visualized by UV light. The flash column chromatography was performed by silica gel Wakogel C-200 (Wako Chemical, Osaka, Japan). The melting points were defined in open capillaries by using MEL-TEMP equipment (Barnstead Thermolyne Corp, Dubuque, IA, USA). UV-VIS spectra were obtained by Lambda 25 UV-VIS (Perkin-Elmer, Waltham, MA, USA) spectrophotometer. IR spectra were recorded in KBr on a Perkin-Elmer spectrophotometer (FT-IR Spectrum BX II). NMR spectra were obtained with Bruker spectrometer (¹H - 400 MHz and ¹³C - 100 MHz) in d-chloroform or d₆-DMSO by using residual solvent signal as an internal standard. HRMS spectra were recorded by Dual-ESI Q-TOF 6520 (Agilent Technologies, Santa Clara, CA, USA) mass spectrometer.