Hepatic lipid extraction was conducted as described before (Folch et al, 1957 (link)). Total cholesterol (Randox Laboratories Ltd.), non-esterified fatty acid (FUJIFILM Wako Chemicals), phospholipid (FUJIFILM Wako Chemicals), and triglyceride (Sigma-Aldrich) levels were determined using commercially available kits according to the manufacturer’s instructions.
Total cholesterol
Manufactured by Randox
Sourced in United Kingdom
The Total Cholesterol test is a laboratory analysis that determines the total amount of cholesterol present in a person's blood sample. Cholesterol is a type of fat that is essential for various bodily functions, but an imbalance can contribute to health issues. The Total Cholesterol test provides a quantitative measurement of the total cholesterol levels, which is a valuable data point for healthcare professionals to assess an individual's overall cardiovascular health.
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Lab products found in correlation
Hydrogen peroxide, by Merck Group (2 mentions)
Bilirubin, by Randox (2 mentions)
Hdl cholesterol, by Randox (2 mentions)
Urea, by Randox (2 mentions)
Creatinine, by Randox (2 mentions)
Ldl cholesterol, by Randox (2 mentions)
Phospholipid, by Fujifilm (1 mentions)
Glutathione (gsh), by Merck Group (1 mentions)
Glutathione gsh 1 chloro 2 4 dinitrobenzene cdnb 5 5 dithiobis 2 nitrobenzoic acid dtnb thiobarbituric acid tba epinephrine, by Merck Group (1 mentions)
Interleukin 6 (il 6), by Diaclone (1 mentions)
Tnf α, by Diaclone (1 mentions)
Adiponectin, by Elabscience (1 mentions)
Total bilirubin, by Randox (1 mentions)
Total protein, by Randox (1 mentions)
Direct bilirubin, by Randox (1 mentions)
Alanine aminotransferase, by Randox (1 mentions)
Alkaline phosphatase, by Randox (1 mentions)
Gamma glutamyl transferase, by Randox (1 mentions)
Mcrp00, by R&D Systems (1 mentions)
Hybri max, by Merck Group (1 mentions)
6 protocols using total cholesterol
1
Hepatic Lipid Extraction and Analysis
2
Albino Rat Biochemical Assays
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Twenty-four albino male rats (Ratus novergicus) with an average weight of 140 g used for this study were purchased from the Animal Holding Unit of the Department of Biochemistry, University of Ibadan, Nigeria. ATR-MET is a product of First Chemical Co Ltd, Changxing, Zhejiang China. GSH, 1-chloro-2, 4-dinitrobenzene (CDNB), 5′, 5′-dithio-bis-2-nitrobenzoic acid (DTNB), thiobarbituric acid (TBA), epinephrine and hydrogen peroxide were purchased from Sigma Chemical Company (London, UK). ALT, AST, ALP, GGT, urea, creatinine, bilirubin, total cholesterol, HDL-cholesterol, LDL-cholesterol and triglycerides kits were obtained from Randox Laboratories Ltd, Antrim UK. All other chemicals and reagent were of analytical grade and were obtained from British Drug House, Poole, UK.
3
Moxifloxacin-Induced Oxidative Stress
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Moxifloxacin hydrochloride (Avelox) was a product of Bayer Healthcare Pharmaceutics, Leverkusen, Germany. Glutathione (GSH), 1-chloro-2,4-dinitrobenzene (CDNB), 5′,5′-dithiobis-2-nitrobenzoic acid (DTNB), thiobarbituric acid (TBA), epinephrine, and hydrogen peroxide were purchased from Sigma Chemical Company (London, UK). Assay kits for alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), γ-glutamyl transferase (γ-GT), urea, creatinine, bilirubin, total cholesterol, HDL-cholesterol, LDL-cholesterol, and triglycerides were products of Randox Laboratories Ltd., Antrim, UK. All other chemicals and reagents used in this study were of analytical grade.
4
Detailed Lipid and Inflammatory Biomarker Analysis
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Commercially available kits were used for the analysis of lipid profile, i.e., total cholesterol (Randox lab ltd., County Antrim, UK), triglycerides, high-density lipoprotein-cholesterol (HDL-C), and low-density lipoprotein- cholesterol (LDL-C) (Agappe diagnostics ltd, Kolkata, India). ELISA kits were used for insulin (Sigma Diagnostics Inc., Livonia, MI, USA), leptin (Diagnostics Biochem Canada Inc., London, ON, Canada), adiponectin (Elabscience Biotechnology Co. Ltd., Wuhan, China), C-reactive protein (CRP, Sigma Diagnostics Inc., Livonia, MI, USA), interleukin-6 (IL-6, Diaclone SAS, Besançon, France), tumor necrosis factor-α (TNF-α, Diaclone SAS, Besançon, France), ferritin (Bio-detect, Laguna Hills, CA, USA) as per assay manual of the kits. Insulin resistance was calculated using McAuley’s Index i.e., exp (2.63–0.28 ln insulin [μU/mL]–0.31 ln triglycerides [mM/mL]) [18 (link)].
5
Comprehensive Biochemical Profiling Protocol
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Total cholesterol, alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), total bilirubin and direct bilirubin, total protein, albumin, urea, creatinine, calcium, sodium, chloride, potassium, and bicarbonate test kits were purchased from Randox Laboratory (United Kingdom).
6
Plasma Cholesterol and CRP Measurement
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Total plasma cholesterol and C-reactive protein (CRP) were measured with commercially available ELISA kits (Total cholesterol, Randox, Crumlin, UK and MCRP00, R&D systems, Minneapolis, MN, USA respectively).
Red blood cells in EDTA whole blood were lysed (red blood cell lysing buffer Hybri-Max, Sigma, St. Louis, MO, USA), followed by the labeling of the remaining leukocytes (monocytes, neutrophils and dendritic, natural killer (NK), natural killer T (NKT) and T-cells). Next, labeled leukocytes were analyzed by flow cytometry as described previously [10 (link)].
Red blood cells in EDTA whole blood were lysed (red blood cell lysing buffer Hybri-Max, Sigma, St. Louis, MO, USA), followed by the labeling of the remaining leukocytes (monocytes, neutrophils and dendritic, natural killer (NK), natural killer T (NKT) and T-cells). Next, labeled leukocytes were analyzed by flow cytometry as described previously [10 (link)].
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