Acetonitrile (hypergrade for LC–MS), formic acid (for LC-MS), and methanol (analytical grade) were purchased from Chromasolv (Bulgaria). The authentic standards gallic, vanillic, protocatechuic, gentisic, salicylic, p-coumaric, rutin, isoquercitrin, luteolin 7-O-rutinoside, luteolin 7-O-glucoside, isorhamnetin 3-O-glucoside, apigenin 7-O-glucoside, luteolin, quercetin, apigenin, and chrysoeriol were obtained from Extrasynthese (Genay, France). Rosmarinic, caffeic, ferulic, chlorogenic, neochlorogenic, 3,4-dicaffeoylquinic, 3,5-dicaffeoylquinic, 1,5-dicaffeoylquinic, and 4,5-dicaffeoylquinic acid were supplied from Phytolab (Vestenbergsgreuth, Germany). Sonchuside A and 8-acetyl-15β-D -glucopyranosyllactucin were previously isolated and identified by Zidorn et al., 2005 [8 (link)].
Vanillic
Manufactured by Extrasynthese
Sourced in France
Vanillic is a laboratory equipment product manufactured by Extrasynthese. It is designed for use in various research and analytical applications. The core function of Vanillic is to provide accurate and reliable measurements. No further details can be provided without extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
Quercetin, by Extrasynthese (1 mentions)
Isoquercitrin, by Extrasynthese (1 mentions)
Apigenin, by Extrasynthese (1 mentions)
Luteolin, by Extrasynthese (1 mentions)
Gentisic, by Extrasynthese (1 mentions)
Protocatechuic, by Extrasynthese (1 mentions)
Gallic, by Extrasynthese (1 mentions)
Chrysoeriol, by Extrasynthese (1 mentions)
Neochlorogenic, by Phytolab (1 mentions)
Caffeic, by Phytolab (1 mentions)
Chlorogenic, by Phytolab (1 mentions)
Isorhamnetin 3 o glucoside, by Extrasynthese (1 mentions)
P coumaric, by Extrasynthese (1 mentions)
3 5 dicaffeoylquinic, by Phytolab (1 mentions)
Luteolin 7 o rutinoside, by Extrasynthese (1 mentions)
3 4 dicaffeoylquinic, by Phytolab (1 mentions)
1 5 dicaffeoylquinic, by Phytolab (1 mentions)
Rosmarinic, by Phytolab (1 mentions)
Luteolin 7 o glucoside, by Extrasynthese (1 mentions)
Rutin, by Extrasynthese (1 mentions)
2 protocols using vanillic
1
HPLC-MS Analysis of Sesquiterpene Lactones
2
Analytical Standards Preparation and Recombinant Enzyme Purification
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Ethanol, hexane and formic acid of analytical reagent grade were from Sigma-Aldrich (Steinheim, Germany). Acetonitrile of HPLC and HPLC-MS grade were purchased from Panreac (Barcelona, Spain). The Milli-Q-system (Millipore SA, Molsheim, France) was used to produce deionized water. Pinoresinol (≥ 95%) and pcoumaric acid (≥ 98%) from Sigma-Aldrich (Steinheim, Germany) were used as standard compounds. Other standards as vanillic, ferulic and syringic acids, Eresveratrol, tyrosol, and kaempferol were purchased from Extrasynthese (France).
Human recombinant PTP-1B was expressed in Escherichia coli TB1 strain, and purified as previously described (Paoli et al., 2013) . 4-nitrophenyl phosphate disodium salt hexahydrate were from Santa Cruz Biotechnology.
Human recombinant PTP-1B was expressed in Escherichia coli TB1 strain, and purified as previously described (Paoli et al., 2013) . 4-nitrophenyl phosphate disodium salt hexahydrate were from Santa Cruz Biotechnology.
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