AAPH was purchased from J&K Scientific (Beijing, China), and different anthocyanins including Pg-3-glu, Dp-3-glu, Cy-3-glu, and Cy-3,5-diglu were purchased from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China), and DPPH was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). The primary antibody for 3β-HSD was purchased from Abcam (Cambridge, UK). Antibodies for StAR and GAPDH were obtained from Cell Signaling Technology (Boston, MA, USA). The secondary anti-rabbit IgG antibody was from Proteintech (Wuhan, China). All other chemicals in this study were of analytical grade unless otherwise stated.
Secondary anti rabbit igg antibody
Manufactured by Proteintech
Sourced in United States
The Secondary anti-rabbit IgG antibody is a laboratory reagent designed to detect and quantify rabbit immunoglobulin G (IgG) in various immunoassays and immunochemistry applications. It is a highly specific and sensitive tool for researchers studying rabbit protein targets.
Automatically generated - may contain errors
Lab products found in correlation
2 2 diphenyl 1 picrylhydrazyl (dpph), by Tokyo Chemical Industry (1 mentions)
P chk1, by Cell Signaling Technology (1 mentions)
γh2ax, by Cell Signaling Technology (1 mentions)
Gsk3α β, by Cell Signaling Technology (1 mentions)
P p70s6k thr421 ser424, by Cell Signaling Technology (1 mentions)
Cyclin d2, by Cell Signaling Technology (1 mentions)
Pgsk3α βser21 9, by Cell Signaling Technology (1 mentions)
P70s6k, by Cell Signaling Technology (1 mentions)
Anti wnt5a, by Thermo Fisher Scientific (1 mentions)
Protoblot 2 ap system, by Promega (1 mentions)
Phenylmethylsulfonyl fluoride, by Beyotime (1 mentions)
Anti sirt3, by Abcam (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Anti β actin, by Abcam (1 mentions)
4 protocols using secondary anti rabbit igg antibody
1
Investigating Antioxidant Mechanisms
2
Comprehensive Western Blotting Procedure for Protein Analysis
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The following antibodies were used for western blotting: DHX9 (Abcam, ab183731), GSK3α/β (Cell Signaling, #5676), p-GSK3α/β-Ser21/9 (Cell Signaling , #8566), P70S6K (Cell Signaling, #2708), p-P70S6K-Thr421/Ser424 (Cell Signaling, #9204), γH2AX(Cell Signaling, #9718), H2AX(Cell Signaling, #7631), p-Chk1 (Cell Signaling, #2348), Chk1 (Cell Signaling, #2360), Cyclin D2 (Cell Signaling, #3741), Myc (Cell Signaling, #5605), β-actin (Share-bio, SB-AB2001), secondary anti-rabbit IgG antibody (Proteintech, SA00001-2), secondary anti-mouse IgG antibody (Proteintech, SA00001-1). The detailed protocol is described in a previous study.8 (link)
3
Wnt5a Protein Expression Analysis
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Chopped tissues and cells were placed in radioimmunoprecipitation assay lysis buffer containing 1% phenylmethylsulfonyl fluoride (Beyotime, Jiangsu, China). Supernatant was collected after 12000 r/min for 15 min. Protein lysates were separated by gel electrophoresis and transferred onto polyvinylidene difluoride membranes. The membranes were exposed to anti-Wnt5a (Invitrogen) or anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies at 4°C overnight. The membrane was incubated in the anti-rabbit IgG secondary antibody (1:5000; Proteintech, Rosemont, IL, United States) for 2 h at room temperature. A ProtoBlot II AP System (Promega, Madison, WI, United States) was used to test the signals. The protein expression was compared with the expression of GAPDH. Densitometry quantified the protein relative expression with ImageJ software (National Institutes of Health, Bethesda, MD, United States).
4
Western Blot Analysis of Sirt3 Protein
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Total protein was extracted using RIPA lysis buffer (Beyotime, Shanghai, China) supplemented with proteinase inhibitors. Protein concentration was determined by a BCA Protein Assay Kit (Beyotime, Shanghai, China). Then, 25 μg of protein sample was separated by 12% SDS-PAGE and transferred onto PVDF membranes (Burlington, MA, USA) by electroblotting, after which nonspecific binding to the membrane was blocked with 5% non-fat milk for 1–2 h at RT. Then, the blotted membranes were probed with anti-Sirt3 (1:2000, Abcam, Cambridge, MA, UK) and anti-β-actin (1:1500, Abcam, Cambridge, MA, UK) antibodies diluted in Tris-buffered saline (TBS) overnight at 4 °C. After incubating with antirabbit IgG secondary antibody (1:2000 dilution, Proteintech, Chicago, IL, USA), protein blots were visualized using an Electro-Chemi-Luminescence detection system (JENE, UK) and quantified with ImageJ software.
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