PCR reactions from the Mesorhizobium strain N33 library were purified using the QIAquick 96 PCR Purification Kit (Qiagen, Mississaugua, ON) and transferred into the 384 master plate format using a Multiprobe II EX Robotic Liquid Handling System (Perkin Elmer, Shelton, CT). Plates were then dried without heat in a DNA 120 Speed Vac (Savant Instruments Inc., Holbrook, NY). Dried samples were resuspended in 10 μl of 3XSSC and stored at −80 °C. A total of 13,056 features, consisting of duplicate spots for each clone and 32 spike-in and negative controls for each block were arranged and printed on Corning GAPS II coated slides, as per Corning instructions. Arrays were printed using the BioRobotics MicroGrid Compact spotter (Digilab Inc, Holliston, MA). The arrays were snap-dried and DNA UV cross-linked to the slides using a CL-1000 ultraviolet cross linker (UPV, Upland, CA) for a total of 300 mJ.
Multiprobe 2 ex robotic liquid handling system
Manufactured by PerkinElmer
Sourced in France
The Multiprobe II EX Robotic Liquid Handling System is a high-precision automated liquid handling platform designed for a wide range of laboratory applications. It features multiple pipetting channels, a temperature-controlled workspace, and advanced software control to ensure accurate and reliable liquid transfers.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using multiprobe 2 ex robotic liquid handling system
1
Microarray Fabrication from Mesorhizobium N33 Library
2
Quantifying CFTR Chloride Channel Activity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CFTR chloride channel activity was evaluated on transfected HeLa cells treated with radioactive iodide ( 125 I) as previously described [13, 14] . The 125 I efflux was realized by the MultiPROBE ® IIex robotic liquid handling system (PerkinElmer Life Sciences, France) and measured by the Packard CobraTMII gamma counter (PerkinElmer Life Sciences, France). Statistical analysis was obtained using GraphPad Software. To compare sets of data, we used Student's t-test. Values were considered as statistically significant for P < 0.05, ns: non-significant difference. In the functional tests, the pTCF-WT is used as a positive control and pTCF-c.1392G > T (p.Lys464Asn) is used as a negative control instead of the pTCF-c.1521_1523delCTT, as it has been demonstrated that the c.1392G > T mutation is a class-II mutation [12] .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!