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6 hydroxynicotine

Manufactured by LGC
Sourced in China

6-hydroxynicotine is a chemical compound that can be used in laboratory settings. It serves as a precursor or intermediate in the synthesis of various organic compounds. The core function of 6-hydroxynicotine is to provide a building block for further chemical reactions and transformations, as required in certain experimental protocols or research applications.

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3 protocols using 6 hydroxynicotine

1

Nicotine Degradation by A. tumefaciens S33

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A. tumefaciens strain S33 was deposited with accession number CCTCC AB 2016054 at the China Type Culture Collection (CCTCC). Nicotine (1 g liter−1) was used as the sole source of carbon and nitrogen to grow strain S33 at 30°C. E. coli strain DH5α and E. coli strain BL21(DE3) were cultured in LB medium at 37°C. NAD+ and NADP+ were purchased from Sigma-Aldrich (Shanghai, China), 6-hydroxynicotine and SAP were purchased from Toronto Research Chemicals, Inc. (Toronto, Canada), and benzaldehyde, furfural, and acetaldehyde were purchased from Macklin (Shanghai, China). Carbenicillin (25 mg liter−1) and kanamycin (50 mg liter−1) were required to culture the E. coli cells.
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2

Nicotine Degradation by A. tumefaciens S33

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A. tumefaciens strain S33 was deposited with accession number CCTCC AB 2016054 at the China Type Culture Collection (CCTCC). Nicotine (1 g liter−1) was used as the sole source of carbon and nitrogen to grow strain S33 at 30°C. E. coli strain DH5α and E. coli strain BL21(DE3) were cultured in LB medium at 37°C. NAD+ and NADP+ were purchased from Sigma-Aldrich (Shanghai, China), 6-hydroxynicotine and SAP were purchased from Toronto Research Chemicals, Inc. (Toronto, Canada), and benzaldehyde, furfural, and acetaldehyde were purchased from Macklin (Shanghai, China). Carbenicillin (25 mg liter−1) and kanamycin (50 mg liter−1) were required to culture the E. coli cells.
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3

Nicotine and Metabolite Standards

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Nicotine, cotinine, and norNicotine were purchased from Sigma-Aldrich (St. Louis, MO) as standards. PseudooxyNicotine, nicotyrine and 6-hydroxyNicotine were obtained from Toronto Research Chemicals (Toronto, ON, Canada). All analytical and high-performance liquid chromatography (HPLC) grade reagents were bought from Merck China (Beijing, China).
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