Ultrapure sodium alginate (FMC Biopolymer), was dissolved in 1% (w/v) d -mannitol aqueous solution (Sigma-Aldrich) at a concentration of 1.5% (w/v). Borax was dissolved in different alginate solutions at 0.59 mM or 1.47 mM concentrations. All alginate solutions were next filtered through a 0.22 μm pore Minisart Syringe Filter (Sarto-rius). For gelation, 2.7 mL of the alginate solutions were mixed with 60 μL of 1.22 M CaSO4·2H2O (Sigma-Aldrich) through two Luer Lock syringe (BS Syringe) connected with a Fluid Dispensing Connector (Braun). Alginate and CaSO4·2H2O were mixed 15 times until complete homogenization. To retard the gelation time, 60 μL of either 0.3 M or 0.5 M Na2HPO4·2H2O (Panreac) were added in the cross-linking reaction.
D mannitol aqueous solution
Manufactured by Merck Group
Sourced in Germany
D-mannitol aqueous solution is a laboratory reagent that consists of the sugar alcohol D-mannitol dissolved in water. It is a colorless, odorless, and viscous liquid. D-mannitol is a commonly used excipient in pharmaceutical formulations and has various applications in analytical chemistry and research settings.
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Lab products found in correlation
2 protocols using d mannitol aqueous solution
1
Alginate Hydrogel Formation with Borax
2
Nanocellulose-Alginate Composite Hydrogel
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Lyophilized sodium alginate powder Pronova SLG100 (NovaMatrix; DuPont, Oslo, Norway) was mixed with sterile 4.6% D-mannitol aqueous solution (Sigma-Aldrich, Darmstadt, Germany) and placed on a shaking table until formation of a transparent alginate hydrogel (3%, w/v). The resulting alginate solution was mixed with 2.5% medical-grade TNC hydrogel TUNICELL ETC (Ocean Tunicell AS) by connecting two syringes with a Luer lock adapter and transferring the solutions back and forth multiple times until a homogeneous nanocellulose-alginate composite hydrogel was formed. The resulting mixture contained 80 vol% of the 2.5% nanocellulose hydrogel and 20 vol% of the 3% alginate hydrogel, which resulted in a ratio between nanocellulose and alginate of 3.33. All materials used in this process were sterile and endotoxinfree.
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