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Anti h3k18ac 39755

Manufactured by Active Motif
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Anti-H3K18ac (39755) is an antibody product offered by Active Motif. It is designed to detect acetylation of lysine 18 on histone H3. This antibody can be used for applications such as chromatin immunoprecipitation (ChIP), western blotting, and immunofluorescence.

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Lab products found in correlation

Ab7311, by Abcam (2 mentions) Anti h3k9ac 61251, by Active Motif (2 mentions) Anti gst sc 459 antibody, by Santa Cruz Biotechnology (2 mentions) Fluorescent secondary antibodies 926 32211, by LI COR (2 mentions) Mononucleosomes, by EpiCypher (2 mentions) Anti flag m2 antibody f1804, by Merck Group (2 mentions) Gateway technique, by Thermo Fisher Scientific (2 mentions) Anti acetyl histone h4 antibody, by Merck Group (2 mentions) Pgex 6p 1 vector, by GE Healthcare (2 mentions)

2 protocols using anti h3k18ac 39755

1

Cloning and Characterization of p300 Domains

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The coding DNA sequences (CDS) encoding human full-length p300 protein and the p300 BD-RING-PHD-HAT-ZZ-TAZ2 region (BRPHZT, amino acids 1035–1830) were cloned into pENTR3C vector and subsequently cloned into p3FLAG and pCDH-FLAG destination vectors using Gateway techniques (Invitrogen), respectively. The CDS encoding the human p300 BD-RING-PHD-HAT-ZZ region (BRPHZ, amino acids 1035–1720) and ZZ domain (amino acids 1650–1720) were cloned into the pGEX-6P-1 vector (GE Healthcare). Point mutations and deletions were generated using a site-directed mutagenesis kit (Stratagene) and verified by Sanger sequencing. Histone peptides bearing different modifications were synthesized at CPC, LLC. Anti-histone antibodies including anti-H3 (Ab1791), anti-H3K4ac (ab176799), anti-H3K9ac (Ab32129), anti-H3K27ac (Ab4729) and anti-H4 (Ab7311) antibodies were obtained from Abcam. Anti-H3K9ac (61251) and anti-H3K18ac (39755) antibodies were from Active Motif. Anti-acetyl-Histone H4 antibody (06–598) was from Millipore. Anti-GST (sc-459) antibody was from Santa Cruz. Anti-FLAG (M2, F1804) antibody was from Sigma. Fluorescent secondary antibodies (926–32211 and 926–68020) were from LI-COR. Mononucleosomes reconstituted from recombinant histones (16–0009) were from EpiCypher.
Zhang Y., Xue Y., Shi J., Ahn J., Mi W., Ali M., Wang X., Klein B.J., Wen H., Li W., Shi X, & Kutateladze T.G. (2018). The ZZ domain of p300 mediates specificity of the adjacent HAT domain for histone H3. Nature structural & molecular biology, 25(9), 841-849.
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2

Cloning and Characterization of p300 Domains

Check if the same lab product or an alternative is used in the 5 most similar protocols
The coding DNA sequences (CDS) encoding human full-length p300 protein and the p300 BD-RING-PHD-HAT-ZZ-TAZ2 region (BRPHZT, amino acids 1035–1830) were cloned into pENTR3C vector and subsequently cloned into p3FLAG and pCDH-FLAG destination vectors using Gateway techniques (Invitrogen), respectively. The CDS encoding the human p300 BD-RING-PHD-HAT-ZZ region (BRPHZ, amino acids 1035–1720) and ZZ domain (amino acids 1650–1720) were cloned into the pGEX-6P-1 vector (GE Healthcare). Point mutations and deletions were generated using a site-directed mutagenesis kit (Stratagene) and verified by Sanger sequencing. Histone peptides bearing different modifications were synthesized at CPC, LLC. Anti-histone antibodies including anti-H3 (Ab1791), anti-H3K4ac (ab176799), anti-H3K9ac (Ab32129), anti-H3K27ac (Ab4729) and anti-H4 (Ab7311) antibodies were obtained from Abcam. Anti-H3K9ac (61251) and anti-H3K18ac (39755) antibodies were from Active Motif. Anti-acetyl-Histone H4 antibody (06–598) was from Millipore. Anti-GST (sc-459) antibody was from Santa Cruz. Anti-FLAG (M2, F1804) antibody was from Sigma. Fluorescent secondary antibodies (926–32211 and 926–68020) were from LI-COR. Mononucleosomes reconstituted from recombinant histones (16–0009) were from EpiCypher.
Zhang Y., Xue Y., Shi J., Ahn J., Mi W., Ali M., Wang X., Klein B.J., Wen H., Li W., Shi X, & Kutateladze T.G. (2018). The ZZ domain of p300 mediates specificity of the adjacent HAT domain for histone H3. Nature structural & molecular biology, 25(9), 841-849.
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