Mlt125 m

Mean systolic blood pressure (SBP) was measured 9 weeks after surgical intervention using a PowerLab 5/20 device coupled to a non-invasive blood pressure controller (both ADInstruments, Oxford, UK) as described previously. Awake mice were placed in a tail vein restrainer and a pressure cuff (MLT125/M, ADInstruments, Oxford, UK) was wrapped around the tail. The mean SBP was calculated from 6–9 measurements per individual animal.

Mean arterial blood pressure was assessed non-invasively using a tail cuff and pulse transducer system (MLT-125 M, ADInstruments, Sydney, Australia) according to the manufacturer’s manual on the restrained mouse. Labchart 7 Pro was used for data acquisition and analysis. For assessment of right ventricular systolic pressure mice were anesthetized using 1.5% isoflurane (Baxter Healthcare, Vienna, Austria), intubated using a 23G venous cannula and mechanically ventilated with a MiniVent type 845 rodent micro-ventilator (Hugo Sachs Elektronik, March-Hugstetten, Germany). Via a bilateral anterolateral thoracotomy a direct right ventricular puncture with a 21G needle and insertion of a micro tip catheter (SPR-1000, Millar Instruments, Houston, TX, USA) were performed.