Multi mode detection synergy h1 model h1mf

A silica gel column (0.040–0.063 mm granule size) and sephadex LH-20 were used for the chromatographic isolation of the extract components. Thin layer chromatography (TLC) analysis was performed on TLC silica gel 60 F254 aluminum sheet 20 × 20 cm (Merck, Darmstadt, Germany). Nuclear magnetic resonance (NMR) spectra were recorded on a Bruker AV400 (USA) spectrometer at 400 MHz for proton and 100 MHz for carbon. Moreover, an Agilent 1260 infinity high performance liquid chromatography instrument via an ESI interface was connected to a 6540 ultrahigh definition accurate mass Q-TOF (Agilent Technologies, Palo Alto, CA, USA). Fourier-transform infrared spectrophotometer (FT-IR), PerkinElmer Spectrum GX (PerkinElmer, Waltham, MA, USA). The absorbance was measured using hybrid Multi-Mode Detection Synergy H1 (Model H1MF) (Bio-TeK Instruments, Winooski, VT, USA). In addition, a McFarland tube densitometer (Grant Instruments DEN-1B, Beaver Falls, PA, USA) and semiautomatic fermentor-biorector–MDFT –N—5 L with mono mode controller (BE Marubishi, Japan) were used.

Thin layer chromatography (TLC) analysis was performed on TLC silica gel 60 F254 aluminum sheet 20 × 20 cm (Merck, Darmstadt, Germany). A silica gel column (0.040–0.063 mm granule size), a sephadex LH-20 column (particle size dry 18–111 µm), and a C18 column (40–63 particle size) were used for chromatographic isolation of the extract constituents. Fourier-transform infrared (FT-IR) spectra were recorded with attenuated total reflectance (ATR) mode on a PerkinElmer spectrum GX (Perkin Elmer, Waltham, MA, USA). Optical rotations were measured using a POLAX-2L polarimeter (Atago, Japan). An Agilent 1260 infinity high performance liquid chromatography instrument via an electrospray ionization (ESI) interface to a 6540 ultrahigh definition accurate mass Q-TOF (Agilent Technologies, Palo Alto, CA, USA) was conducted. Nuclear magnetic resonance (NMR) spectra were recorded on a Bruker AV400 (Bruker, Billerica, MA, USA) spectrometer at 400 MHz for proton and 100 MHz for carbon. The absorbance was measured using hybrid Multi-Mode Detection Synergy H1 (Model H1MF) (Bio-TeK Instruments, Winooski, VT, USA). The high performance liquid chromatography (HPLC) was performed using Agilent Technology (model 1260 infinity with fraction collector, Santa Clara, CA, USA).