On the 8th day, blood samples were collected for the estimation of oxidative stress parameters such as nitric oxide (NOx), malondialdehyde (MDA), total oxidative stress (TOS), and antioxidant parameters such as catalase (CAT) and total antioxidant capacity (TAC). Matrix metalloproteinases (MMP-2 and MMP-9), renal function (creatinine, blood urea nitrogen (BUN), and urea) were also assessed. At the end of the experiment, all the animals were euthanized after general anesthesia (ketamine—5 mg/kg body weight, i.p. route) [47 (link)] by cervical dislocation, after the blood sample collection from retro-orbital plexus.
A Jasco V-350 UV-VIS spectrophotometer (Jasco International Co, Ltd., Tokyo, Japan) was used for all measurements. The oxidative stress intensity (NOx, MDA, and TOS) and antioxidant capacity of plasma (CAT and TAC) parameters were measured by the methods described somewhere else [48 (link)]. Metalloproteinases assessment was made with a Stat Fax 303 ELISA reader (Quantikine, McKinley Place NE, MN, USA), using a rat ELISA kit (Boster Biological Technology, Pleasanton, CA, USA). Renal function parameters assessment was made by colorimetric methods [49 (link),50 (link)], and the reagents were purchased from Spinreact (Girona, Spain).
A Jasco V-350 UV-VIS spectrophotometer (Jasco International Co, Ltd., Tokyo, Japan) was used for all measurements. The oxidative stress intensity (NOx, MDA, and TOS) and antioxidant capacity of plasma (CAT and TAC) parameters were measured by the methods described somewhere else [48 (link)]. Metalloproteinases assessment was made with a Stat Fax 303 ELISA reader (Quantikine, McKinley Place NE, MN, USA), using a rat ELISA kit (Boster Biological Technology, Pleasanton, CA, USA). Renal function parameters assessment was made by colorimetric methods [49 (link),50 (link)], and the reagents were purchased from Spinreact (Girona, Spain).