Samples were prepared and detected as described [49 (link)]. The antibodies used for Western blots were: mouse anti-α-tubulin (clone B-5-1-2), mouse anti-SR proteins (clone 1H4), and rabbit anti-HA from Sigma-Aldrich; rabbit anti-RAC1B from Millipore; mouse anti-PCNA from MerckBiosciences; mouse anti-SRPK1 and mouse anti-β-catenin from BD Transduction Laboratories; rabbit anti-AKT, rabbit anti-GSK3β (27C10) and rabbit anti-PhosphoGSK3β (Ser9) (D85E12) from Cell Signalling; mouse anti-p-GSK-3α/β (6D3) (sc-81496), mouse anti-SF2/ASF (96) (sc-33652) (SRSF1) and anti-Histone H2b (sc-10808) from Santa Cruz Biotechnology and sheep anti-WNK1 from Dundee University. For the detection of phospho-SRSF1, total SRSF1 was first immunoprecipitated with the specific anti-SRSF1 antibody (sc-33652) and this enriched fraction then probed with the-SR phosphoepitope antibody (1H4). For densitometric analysis of band intensities, X-ray films from at least three independent experiments were digitalized and analyzed using ImageJ software (NIH).
Mouse anti srpk1 and mouse anti β catenin
Manufactured by BD
Mouse anti-SRPK1 and mouse anti-β-catenin are laboratory reagents used for the detection and analysis of the target proteins SRPK1 and β-catenin in biological samples. These antibodies are specific for the respective target proteins and can be used in various immunoassay techniques, such as Western blotting, immunohistochemistry, and immunoprecipitation, to identify and quantify the presence of SRPK1 and β-catenin in cells and tissues.
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Lab products found in correlation
2 protocols using mouse anti srpk1 and mouse anti β catenin
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Antibody Characterization for Western Blot Analysis
2
Antibody Detection and Validation
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Samples were prepared and detected as described (Matos and Jordan 2006 (link)). The antibodies used for Western blots were: mouse anti-α-tubulin (clone B-5-1-2) from Sigma; T7-Tag Antibody from Novagen/VWR; rabbit anti-Rac1b from Millipore; mouse anti-SR proteins (clone 1H4), mouse anti-ASF/SF2 (SRSF1) and mouse anti-SRp20 (SRSF3) from Invitrogen; mouse anti-SRPK1 and mouse anti-β-catenin from BD Transduction Laboratories; rabbit anti-AKT and rabbit anti-GSK3β (27C10) from Cell Signalling; mouse anti-cyclin E (ab3927) from Abcam; and anti-Histone H2b (sc-10808) from Santa Cruz Biotechnology. Shown are Western blot images that are representative of at least three independent experiments.
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