Log In Sign up
The largest database of trusted experimental protocols

Annexin 5 conjugates for apoptosis detection kit

Manufactured by Thermo Fisher Scientific
Visit Supplier

The Annexin V Conjugates for Apoptosis Detection kit is a laboratory product designed to detect apoptosis, a form of programmed cell death. The kit contains fluorescently labeled Annexin V, a protein that binds to phosphatidylserine, a molecule that is exposed on the surface of cells undergoing apoptosis. This allows for the identification and quantification of apoptotic cells using flow cytometry or microscopy techniques.

Automatically generated - may contain errors

Lab products found in correlation

Click it edu, by Thermo Fisher Scientific (2 mentions) Facscanto 2 flow cytometer, by BD (1 mentions)

Close spelling variants of this product

Annexin V Conjugates for Apoptosis Detection Annexin V Apoptosis Detection Kit Annexin V Conjugate Annexin V apoptosis kit Annexin V detection kit Apoptosis detection kit Annexin V kit Annexin V

3 protocols using annexin 5 conjugates for apoptosis detection kit

1

Quantifying Cellular Proliferation and Apoptosis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were incubated with 10 µM EdU (Click-iT EdU) (Thermo Fisher Scientific, C10634) in medium for 4 h at 5% O2/5% CO2/37 °C. The 3D clusters were prepared for whole-mount immunostaining as described above. Dissociated cells were collected for flow cytometry as described above. Permeabilization, blocking and Click-iT reaction for EdU detection were performed according to the manufacturer’s instructions. Immunostaining of EdU-labelled 3D clusters were performed with antibodies supplied with the kit and with DAPI (1 μg ml−1) for nuclear staining. Flow cytometry of EdU-labelled dissociated cells was performed with DAPI (10 μg ml−1) staining cells for DNA content. Cell apoptosis was measured by Annexin V Conjugates for Apoptosis Detection kit (Thermo Fisher Scientific, A13202) according to the manufacturer’s instructions.
Wong Y.F., Kumar Y., Proks M., Herrera J.A., Rothová M.M., Monteiro R.S., Pozzi S., Jennings R.E., Hanley N.A., Bickmore W.A, & Brickman J.M. (2023). Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation. Nature Cell Biology, 25(3), 481-492.
+ Open protocol
+ Expand
2

Measuring Cell Proliferation and Apoptosis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were incubated with 10 μM EdU (Click-iT EdU) (Thermo Fisher Scientific, C10634) in medium for 4 hours at 5% O2/5% CO2/37°C. The 3D clusters were prepared for whole mount immunostaining as described above. Dissociated cells were collected for flow cytometry as described above. Permeabilization, blocking and Click-iT reaction for EdU detection were performed according to the manufacturer’s instructions. Immunostaining of EdU-labeled 3D clusters were performed with antibodies supplied with the kit and with DAPI (1 μg/mL) for nuclear staining. Flow cytometry of EdU-labeled dissociated cells was performed with DAPI (10 μg/mL) staining cells for DNA content. Cell apoptosis was measured by Annexin V Conjugates for Apoptosis Detection kit (Thermo Fisher Scientific, A13202) according to the manufacturer’s instructions.
Minter R.M., Ferry M.A., Rectenwald J.E., Bahjat F.R., Oberholzer A., Oberholzer C., La Face D., Tsai V., Ahmed C.M., Hutchins B., Copeland EM I.I.I., Ginsberg H.S, & Moldawer L.L. (2000). Extended lung expression and increased tissue localization of viral IL-10 with adenoviral gene therapy. Proceedings of the National Academy of Sciences of the United States of America, 98(1), 277-282.
+ Open protocol
+ Expand
3

Apoptosis Detection by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cells were inoculated into 6 wells (3 × 105 cells in culture medium/well). After 2 days of transfection, the cells were trypsinized and stained with Annexin V Conjugates for Apoptosis Detection kit (Thermo Fisher Scientific, Cat. A13201). The apoptotic cells in each group were detected with the FACSCanto II flow cytometer (BD Biosciences). The experiment was repeated three times in each group.
Chen Y.C., Chen C.Y., Chiang T.W., Chan M.H., Hsiao M., Ke H.M., Tsai I.J, & Chuang T.J. (2023). Detecting intragenic trans-splicing events from non-co-linearly spliced junctions by hybrid sequencing. Nucleic Acids Research, 51(15), 7777-7797.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!