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Af2910 sp

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AF2910-SP is a recombinant human Activin A Receptor Type 1 Fc Chimera. It is a fusion protein composed of the extracellular domain of the human Activin A Receptor Type 1 and the Fc portion of human immunoglobulin G1.

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Lab products found in correlation

Lsm 780 confocal microscope, by Zeiss (6 mentions) Anti fade prolong gold, by Thermo Fisher Scientific (2 mentions) Rabbit anti nur77, by Proteintech (2 mentions)

Close spelling variants of this product

AF2910

2 protocols using af2910 sp

1

Immunohistochemistry of Mouse Femur

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Freshly dissected femur from female C57BL/6 wild-type mouse was fixed in 4% paraformaldehyde overnight followed by decalcification in 10% EDTA for 1 week. Samples were cut in 5-μm-thick longitudinally oriented sections. After deparaffinize and antigen retrieval, sections were blocked in PBS with 5% bovine serum albumin (BSA) for 1 hour and then stained overnight with goat-anti-Alpl (R&D: AF2910-SP, 10 μg/mL). Rabbit-anti-Nur77 (Proteintech:12235-1-AP, 1:2000) was used as secondary antibodies (from Invitrogen, 1:400). Slides were mounted with anti-fade prolong gold (Invitrogen) and images were acquired with a Zeiss LSM780 confocal microscope.
Gong Y., Yang J., Li X., Zhou C., Chen Y., Wang Z., Qiu X., Liu Y., Zhang H., Greenbaum J., Cheng L., Hu Y., Xie J., Yang X., Li Y., Bai Y., Wang Y.P., Chen Y., Tan L.J., Shen H., Xiao H.M, & Deng H.W. (2021). A systematic dissection of human primary osteoblasts in vivo at single-cell resolution. Aging (Albany NY), 13(16), 20629-20650.
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2

Immunohistochemical Analysis of Alpl and Nur77 in Mouse Femur

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Freshly dissected femur from female C57BL/6 mouse was fixed in 4% paraformaldehyde overnight followed by decalcification in 10% EDTA for 1 week. Samples were cut in 5-µm-thick longitudinally oriented sections. After deparaffinize and antigen retrieval, sections were blocked in PBS with 5% bovine serum albumin (BSA) for 1 hour and then stained overnight with goatanti-Alpl (R&D: AF2910-SP, 10 µg/mL). Rabbit-anti-Nur77 (Proteintech:12235-1-AP, 1:2000) was used as secondary antibodies (from Invitrogen, 1:400). Slides were mounted with anti-fade prolong gold (Invitrogen) and images were acquired with a Zeiss LSM780 confocal microscope.
Gong Y., Yang J., Li X., Zhou C., Chen Y., Wang Z., Qiu X., Liu Y., Zhang H., Greenbaum J., Cheng L., Hu Y., Xie J., Yang X., Li Y., Bai Y., Wang Y., Chen Y., Tan L., Shen H., Xiao H., & Deng H. (2020). A systematic dissection of human primary osteoblastsin vivoat single-cell resolution.
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