Log In Sign up
The largest database of trusted experimental protocols

Female c57bl 6 b6 and tcr δ mice

Manufactured by Jackson ImmunoResearch
Visit Supplier
Sourced in Montenegro

Female C57BL/6 (B6) and TCR-δ−/− mice are laboratory animals commonly used in biomedical research. C57BL/6 mice are a widely used inbred strain, while TCR-δ−/− mice are genetically modified to lack the T cell receptor delta chain. These mouse models provide researchers with tools to study various biological processes and disease mechanisms.

Automatically generated - may contain errors

Lab products found in correlation

Il 23, by R&D Systems (3 mentions) Recombinant murine il 1, by R&D Systems (2 mentions) 50 n ethylcarboxamidoadenosine neca, by Merck Group (2 mentions) 2 p 2 carboxyethyl phenethylamino 5 n ethylcarboxamidoadenosine cgs21680, by Merck Group (2 mentions) Bay 60 6538, by Merck Group (2 mentions) Interleukin 7 (il 7), by R&D Systems (2 mentions) Mrs1754, by Merck Group (1 mentions) Sch58261, by Merck Group (1 mentions) Recombinant murine il 12, by R&D Systems (1 mentions) Mouse ifn γ, by BioLegend (1 mentions) Gm csf, by R&D Systems (1 mentions)

Close spelling variants of this product

C57BL/6 (B6) female mice C57BL/6 (B6) mice C57BL/6 females C57BL/6 (B6) C57BL/6 mice Δsµ mice C57BL/6

3 protocols using female c57bl 6 b6 and tcr δ mice

1

Adenosine Receptor Regulation of T Cell Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols
Female C57BL/6 (B6) and TCR-δ-/- mice on the B6 background, purchased from Jackson Laboratory (Bar Harbor, ME), were housed and maintained in the animal facilities of the University of Southern California. Recombinant murine IL-1, IL-7, and IL-23 were purchased from R & D (Minneapolis, MN). Fluorescein isothiocyanate (FITC)-, phycoerythrin (PE)-, or allophycocyanin (APC)-conjugated antibodies (Abs) against mouse CD73, CD44, αβ T cell receptor (TCR), or γδ TCR and their isotype control antibodies were purchased from Biolegend (San Diego, CA). (PE)-conjugated anti-mouse A2AR monoclonal antibody was purchased from Santa Cruz Biotechnology (Dallas, Texas). The non-selective AR agonist 50-N-ethylcarboxamidoadenosine (NECA), selective A2AR agonist 2-p-(2-carboxyethyl) phenethylamino-5′-N-ethylcarboxamidoadenosine (CGS21680), selective A2AR antagonist (SCH 58261), selective A2BR agonist (BAY 60-6538), and selective A2BR antagonist (MRS 1754), and the CD73 inhibitor α,β-methylene ADP (APCP) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Liang D., Zuo A., Shao H., Chen M., Kaplan H.J, & Sun D. (2014). Roles of the Adenosine Receptor and CD73 in the Regulatory Effect of γδ T Cells. PLoS ONE, 9(9), e108932.
+ Open protocol
+ Expand
2

Murine Immune Cell Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Female C57BL/6 (B6) and TCR-δ−/− mice on the B6 background, purchased from Jackson Laboratory (Bar Harbor, ME), were housed and maintained in the animal facilities of the University of Southern California. Recombinant murine IL-1, IL-7, and IL-23 were purchased from R & D (Minneapolis, MN). Fluorescein isothiocyanate (FITC)-, or phycoerythrin (PE)-conjugated antibodies against the mouse αβ T cell receptor (αβ TCR), γδ TCR, IL-17, IFNγ, or CD44 and isotype control antibodies were purchased from Biolegend (San Diego, CA). The non-selective AR agonist 50-N-ethylcarboxamidoadenosine (NECA), the selective A2AR agonist 2-p-(2-carboxyethyl) phenethylamino-5′-N-ethylcarboxamidoadenosine (CGS21680), and the selective A2BR agonist (BAY 60-6538) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Liang D., Zuo A., Shao H., Chen M., Kaplan H.J, & Sun D. (2014). The anti- or pro-inflammatory effect of an adenosine receptor agonist on the Th17 autoimmune response is inflammatory environmental-dependent. Journal of immunology (Baltimore, Md. : 1950), 193(11), 5498-5505.
+ Open protocol
+ Expand
3

Murine Immune Cell Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Female C57BL/6 (B6) and TCR-δ−/− mice on the B6 background, purchased from Jackson Laboratory (Bar Harbor, ME), were housed and maintained in the animal facilities of the University of California, Los Angeles, and were used at 12–16 weeks of age. The experimental protocols were approved by the Institutional Animal Care and Use Committee of the University of California, Los Angeles (Protocol number: ARC#2014-029-03A). Recombinant murine IL-12, IL-23, and GM-CSF were purchased from R & D Systems (Minneapolis, MN). FITC-, PE-, or APC-conjugated mouse monoclonal antibodies against mouse αβ T cell receptor (TCR, clone H57–597), mouse γδ TCR-Vγ4 (UC3), mouse γδ TCR-Vγ1 (211), mouse γδ TCR (clone GL3), mouse IL-17, mouse IFN-γ, mouse CD73, or mouse CD44 and isotype control antibodies were all purchased from BioLegend (San Diego, CA).
Liang D., Nian H., Shao H., Kaplan H.J, & Sun D. (2017). Functional conversion and dominance of γδ T subset in mouse experimental autoimmune uveitis. Journal of immunology (Baltimore, Md. : 1950), 198(4), 1429-1438.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!