2 morpholinoethanesulfonicacid mes

Manufactured by Dojindo Laboratories

Sourced in Japan

2-morpholinoethanesulfonicacid (MES) is a chemical compound commonly used as a buffer in various biological and biochemical applications. It helps maintain a stable pH environment for optimal performance of enzymes, proteins, and other biomolecules.

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Lab products found in correlation

Ms salt, by Merck Group (1 mentions) Sucrose, by Fujifilm (1 mentions) Agarose, by Fujifilm (1 mentions) Potassium hydroxide koh, by Fujifilm (1 mentions) Bovine serum, by Thermo Fisher Scientific (1 mentions) Rabbit serum, by Thermo Fisher Scientific (1 mentions) Alkaline phosphatase labeling kit nh2, by Dojindo Laboratories (1 mentions) Nitrocellulose membrane ff120 hp, by GE Healthcare (1 mentions) 5 bromo 4 chloro 3 indolyphosphate p toluidine salt bcip, by Roche (1 mentions) N hydroxysuccinimide, by GE Healthcare (1 mentions) 1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride, by GE Healthcare (1 mentions) Colloidal gold, by BBI Solutions (1 mentions) Tris hydroxymethyl aminomethane tris, by Nacalai Tesque (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Glutaraldehyde solution, by Fujifilm (1 mentions) Glucose oxidase gox from aspergillus niger, by Toyobo (1 mentions) 3 morpholinopropanesulfonic acid mops, by Dojindo Laboratories (1 mentions) Poly l lysine hydrobromide, by Fujifilm (1 mentions) Poly allylamine hydrochloride, by Merck Group (1 mentions) Streptomycin sulfate, by Fujifilm (1 mentions)

4 protocols using 2 morpholinoethanesulfonicacid mes

Arabidopsis thaliana Seed Sterilization and Growth

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The seeds of
wild-type Arabidopsis thaliana (A. thaliana) were sterilized in ethanol/water (70%
v/v) for 1 min and then twice in bleach/water (5% v/v) for 4 min and
then rinsed six times with sterilized water. After that, the seeds
were stored at 4 °C for at least 2 days. The seeds were grown
on half-strength Murashige and Skoog (1/2 MS) medium containing MS
salts (Sigma-Aldrich, St. Louis, MO) (2.2 g/L), 2-morpholinoethanesulfonic
acid (MES) (Dojindo Molecular Technologies, Inc., Kumamoto, Japan)
(0.5 g/L, adjusted to pH 5.7 with potassium hydroxide (KOH) (FUJIFILM
Wako Pure Chemical)), sucrose (FUJIFILM Wako Pure Chemical) (10 g/L),
and agarose (FUJIFILM Wako Pure Chemical) (0.8% w/v) under daylength
conditions of 16 h of light/8 h of darkness at 22 °C. Seedlings
were harvested 6 days after germination and used for all experiments.

Abe N., Fujita S., Miyamoto T., Tsuchiya K, & Numata K. (2023). Plant Mitochondrial-Targeted Gene Delivery by Peptide/DNA Micelles Quantitatively Surface-Modified with Mitochondrial Targeting and Membrane-Penetrating Peptides. Biomacromolecules, 24(8), 3657-3665.

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Immunoassay Reagents and Materials Protocol

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The reagents used in this study were as follows: Nitrocellulose membrane FF120 HP, glass filter, 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC), and N-hydroxysuccinimide (NHS) purchased from GE Healthcare (IL, USA); casein and NaN3 purchased from Kanto Chemicals (Tokyo, Japan); Tris (hydroxymethyl) aminomethane (Tris) and N-cyclohexyl-3-aminopropanesulfonic acid (CAPS) purchased from Nacalai Tesque (Kyoto, Japan); and absorption pad and Bovine serum albumin (BSA) bought from Merck (NJ, USA). The backing sheet was obtained from Nippn Engineering (Tokyo, Japan). Colloidal gold was purchased from BBI Solutions, (Newport, UK). A microtiter plate (96 well) was obtained from Greiner (Frickenhausen, Germany). Alkaline Phosphatase Labeling Kit-NH2, 3-[(3-Cholamidopropyl) dimethylammonio]-propanesulfonate (CHAPS), and 2-morpholinoethanesulfonic acid (MES) were purchased from Dojindo (Kumamoto, Japan).
5-Bromo-4-chloro-3-indolyphosphate p-toluidine salt (BCIP) was purchased from Roche Diagnostics (Basel, Switzerland). Colored cellulose particles (NanoAct TM ) were obtained from Asahi Kasei (Tokyo, Japan). Bovine serum and rabbit serum were purchased from Thermo Fisher Scientific (Tokyo, Japan). Other reagents were obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan).

Harada Y., Ohmuro-Matsuyama Y., Tsuna M, & Ueda H. (2021). An Open Sandwich Immunochromatography for Non-competitive Detection of Small Antigens. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 37(3).

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Glucose Oxidase Enzyme Characterization

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Glucose oxidase (GOx) from Aspergillus niger was purchased from Toyobo (Tokyo, Japan). We determined the concentration of GOx spectrophotometrically using the absorption coefficient of 18240 M -1 cm -1 at 460 nm. 11 ε-Poly-L-lysine was kindly donated by JNC (Tokyo). The sample consisted mainly of 25 -35 L-lysine residues, and its weight-average molecular weight was reported to be 4700 Da. 9, 12 Poly-L-lysine hydrobromide (αPL, MW = 15000 -30000), glycol chitosan (GCh), potassium ferrocyanide, hydroquinone (HQ), D-glucose, and 25% glutaraldehyde solution were purchased from Wako Pure Chemical Industries (Osaka, Japan). Bovine serum albumin (BSA) and poly(allylamine hydrochloride) (PAA, MW = 15000) were purchased from Sigma-Aldrich (St. Louis, MO). 2-Morpholinoethanesulfonic acid (MES) and 3-morpholinopropanesulfonic acid (MOPS) were purchased from Dojindo Laboratories (Mashiki, Japan). They were used without further purification. Other chemicals were of reagent grade and were used as received.

Uematsu K., Ueno T, & Katano H. (2018). Effect of ε-Poly-L-lysine on a Glucose Sensor Based on Glucose Oxidase and Ferricyanide Ion. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 34(8).

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Neomycin Production by Streptomyces fradiae

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Neomycin trisulfate was obtained from Aldrich. Tobramycin sulfate (2.5H2SO4) and amikacin disulfate were obtained from Nacalai Tesque. Gentamycin sulfate (2.5H2SO4) was obtained from Panreac Applichem.
Kanamycin monosulfate and streptomycin sulfate (1.5H2SO4) were obtained from Wako. These were used as standard materials. Amaranth (Na3AR) was obtained from Aldrich. Tetraphenylborate sodium salt (NaTPB), 2-morpholino-ethanesulfonic acid (MES), and N-tris-(hydroxymethyl)methyl-3-aminopropanesulfonic acid (TAPS) were obtained from Dojindo. These were used without further purification. Other chemicals were of reagent grade, and were used as received.
The production of NEO by Streptomyces fradiae NBRC12773 was performed using a slight modification of the previously described method. 15 S. fradiae NBRC12773 was cultivated in culture medium containing 2% (w/w) starch, 0.5% glucose, 0.5% yeast extract, 0.3% peptone, 0.3% meat extract, 0.02% KH2PO4, 0.06% MgSO4•7H2O, and 0.1% CaCO3 for 5 days at 28°C with rotary shaking. The culture broth was centrifuged, and proteins and non-polar compounds were removed from the resulting supernatant using chloroform extraction. The resulting sample was then subjected to further experiments.

Katano H., Kuroda Y., Taira S., Maruyama C, & Hamano Y. (2017). Colorimetric Microtiter Plate Assay of Polycationic Aminoglycoside Antibiotics in Culture Broth Using Amaranth. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 33(4).

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