12-hydroxystearic acid, methyl ricinoleate, oleoyl chloride, linoleoyl chloride, dry pyridine, anhydrous dichloromethane, CDCl3, and 4-dimethylaminopyridine were purchased from Tokyo Chemical Industry (Tokyo, Japan). Dicyclohexyl carbodiimide, lithium hydroxide, Methanol for LC/MS, and all other reagents of synthetic grade were obtained from Wako Pure Chemical Corporation (Tokyo, Japan). Docosahexaenoic acid and Eicosapentaenoic acid with purity ≥98%, were purchased from Cayman Chemicals (Ann Arbor, MA, USA). Thin-layer chromatography (TLC) Silica gel 60G F254 glass plates (20 × 20 cm) were obtained from Merck (Tokyo, Japan) and spots were visualized by spraying 5% methanolic H2SO4. The column chromatography was performed by using spherical type Silica Gel N60 of particle size 40–50 μm, obtained from Kanto Chemical Industry (Tokyo, Japan). 1H and 13C NMR spectra were acquired using 400 MHz JNM-ECX400P (JEOL, Japan). The spectra were processed using ACD/NMR software and the chemical shifts(δ) values are given in ppm. The accurate mass measurements were performed by LTQ Orbitrap XL (Thermo Fisher scientific). Low-resolution electrospray ionization mass spectra (LR-ESI-MS) was recorded by LXQ (Thermo Fisher scientific), The methyl ester of 12-hydroxystearic acid (12-HSA) was prepared by direct methylation 12-HSA by refluxing at 80 °C in methanolic 2N HCl.
Cdcl3
Manufactured by Tokyo Chemical Industry
Sourced in Japan
CDCl3 is a deuterated chloroform compound commonly used as a solvent in nuclear magnetic resonance (NMR) spectroscopy. It serves as a medium for dissolving and analyzing various chemical samples during NMR analysis.
Automatically generated - may contain errors
Lab products found in correlation
Jnm ecx400p, by JEOL (2 mentions)
Ltq orbitrap xl, by Thermo Fisher Scientific (2 mentions)
Methyl ricinoleate, by Tokyo Chemical Industry (1 mentions)
Eicosapentaenoic acid, by Cayman Chemical (1 mentions)
Docosahexaenoic acid, by Cayman Chemical (1 mentions)
Anhydrous dichloromethane, by Tokyo Chemical Industry (1 mentions)
Lithium hydroxide, by Fujifilm (1 mentions)
Dry pyridine, by Tokyo Chemical Industry (1 mentions)
Dicyclohexyl carbodiimide, by Fujifilm (1 mentions)
12 hydroxystearic acid, by Tokyo Chemical Industry (1 mentions)
Silica gel 60g f254 glass plates, by Merck Group (1 mentions)
4 dimethylaminopyridine, by Tokyo Chemical Industry (1 mentions)
Trifluoroacetic acid, by Tokyo Chemical Industry (1 mentions)
Sodium methoxide, by Tokyo Chemical Industry (1 mentions)
Precoated plates, by Merck Group (1 mentions)
Acd nmr processor software, by Advanced Chemistry Development (1 mentions)
Cd3od, by Cambridge Isotopes (1 mentions)
Silica gel 60 f254, by Merck Group (1 mentions)
2 protocols using cdcl3
1
Synthesis and Characterization of Hydroxy Fatty Acid Derivatives
2
Synthesis and Characterization of Phospholipid Derivative
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Dipalmitoyl-sn-glycero-3-phosphoethanolamine, CDCl3, trifluoroacetic acid, trityl bromide, and sodium methoxide were obtained from Tokyo Chemical Industry (Tokyo, Japan), CD3OD is obtained from Cambridge Isotope Laboratories, Inc.,. All other reagents of synthetic grade and LC-MS grade (methanol) were purchased from Wako Pure Chemical Corporation (Tokyo, Japan). TLC was performed on Merck pre-coated plates (20 cm × 20 cm; layer thickness, 0.25 mm; Silica Gel 60F254); the spots were visualized by spraying Ninhydrin in ethanol or 5% H2SO4 in methanol when applicable. Silica Gel N60 (spherical type, particle size 40-50 μm; Kanto Chemical Industry) was used for column chromatographic purification. Proton and carbon NMR was recorded with 400 MHz JNM-ECX400P (JEOL, Japan; 1 H: 400 MHz, 13 C: 100 MHz); multiplicities are given as singlet (s), broad (br), doublet (d), double doublets (dd), triple doublets (td), triplet (t), quintet (q), or multiplet (m). Chemical shifts are given in ppm. 1 H NMR spectra were processed by ACD/NMR processor software (Advanced Chemistry Development, inc.). High-resolution electrospray ionization mass spectra (HR-ESI-MS) was recorded by Linear trap quadrupole (LTQ) Orbitrap XL (Thermo Fisher scientific). Lowresolution electrospray ionization mass spectra (LR-ESI-MS) was recorded by LXQ (Thermo Fisher scientific).
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