Mda mb 453

T47D, MDA-MB-453, MDA-MB-486, BT549 and CAL-51 cells were purchased from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). MCF7 cells were purchased from ATCC. HCC1806 and HCC1937 cells were kind gifts of Rene Bernards, Amsterdam. SUM159 cells were a kind gift of Volker Haucke, Berlin. MCF7 and T47D cells were cultured in DMEM (Life Technologies) supplemented with 10% FBS, 5 μg/mL insulin (Sigma, cat.# I0516) and 1% penicillin/streptomycin (Sigma). MDA-MB-453, MDA-MB-468, BT549 and CAL-51 cells were cultured in DMEM supplemented with 10% FBS, 1% non-essential amino acids (Gibco, 11140050) and 1% penicillin/streptomycin. HCC1806 and HCC1937 cells were cultured in RPMI-1640 (Life Technologies) supplemented with 10% FBS and 1% penicillin/streptomycin. SUM159 cells were cultured in Ham/F12 (Life Technologies), 10% FCS, 1 μg/ml hydrocortisone, 10 mM HEPES (Gibco, 11560496), 5 μg/ml insulin and 1% penicillin/streptomycin. All cell lines were cultured at 37 °C and 5% CO₂ in a humidified incubator. See Additional file 1: Table S1 for a summary on cell line characteristics [41 , 42 ].

HCC1806, MDA-MB-436, HCC1395, ZR-75-1, T47D, MCF7, BT474, and MCF10A were a kind gift from CRUK Manchester Institute and they were authenticated by short tandem repeat profiling by CRUK MI core facility unit. HCC1806 was authenticated again in August 2017 by ATCC Service. HCC1569 and HCC1599 were purchased from ATCC in April 2016. MDA-MB-453 and CAL-85-1 were purchased from DSMZ in November 2014 and November 2016, respectively. MDA-MB-321 and BT20 was a kind gift from Department of Immunology, Medical University of Warsaw (purchased from ATCC in February 2018). ATCC and DSMZ authenticate cell lines by short tandem repeat profiling. HCC1806, HCC1395, BT474, BT20, ZR-75-1, T47D, HCC1599 and HCC1569 were cultured in RPMI-1640 supplemented with 10% FCS, 1% penicillin/streptomycin, 2 mM l-glutamine and 1 mM sodium pyruvate. MCF7, CAL-85-1, MDA-MB-453 and MDA-MB-231 were cultured in DMEM supplemented with 10% FCS, 1% penicillin/streptomycin, 2 mM l-glutamine and 1 mM sodium pyruvate. MDA-MB-436 were cultured in RPMI-1640 with 25 mM HEPES supplemented with 10% FCS, 1% penicillin/streptomycin, 2 mM l-glutamine, 1 mM sodium pyruvate and 10 µg/ml insulin. MCF10A were grown in DMEM/F12 supplemented with 5% horse serum, 20 ng/ml EGF, 0.5 mg/ml hydrocortisone, 100 ng/ml cholera toxin, 10 µg/ml insulin, 1% penicillin/streptomycin.