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Anti hcmv capsid mcp

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The Anti-HCMV capsid MCP is a laboratory tool used for the detection and analysis of human cytomegalovirus (HCMV) capsid proteins. It is a specific antibody that binds to the major capsid protein (MCP) of HCMV. This product can be used in various immunoassay techniques to identify and quantify the presence of HCMV capsid proteins in samples.

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Lab products found in correlation

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2 protocols using anti hcmv capsid mcp

1

EV and HCMV Protein Characterization

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Total proteins were extracted from both EV and HCMV fractions, according to RIPA Lysis and Extraction Buffer (Thermo Fisher Scientific, Waltham, MA). 10 μg of proteins were loaded on a 4–20% precast polyacrylamide gel (Bio-Rad Laboratories, Hercules, CA) and separated by SDS-PAGE, then transferred to PVDF membranes and probed with anti-CD63 (1 μg/ml, Thermo Fisher Scientific, Waltham, MA), anti-Calnexin (1 μg/ml, Thermo Fisher Scientific, Waltham, MA), anti-Rab27A (1 μg/ml, Thermo Fisher Scientific, Waltham, MA), anti-HCMV capsid MCP (2 μg/ml), and anti-HCMV tegument pp150 (2 μg/ml) monoclonal primary anti-mouse monoclonal antibodies (clones 28–4 and 36–14) and then goat peroxidase-conjugated anti-mouse IgG secondary anti-body (Bio-Rad Laboratories, Hercules, CA). Peroxidase activity and digital images were detected by using V3 Western Workflow™ (Bio-Rad Laboratories, Hercules, CA).
Zicari S., Arakelyan A., Palomino R.A., Fitzgerald W., Vanpouille C., Lebedeva A., Schmitt A., Bomsel M., Britt W, & Margolis L. (2018). Human cytomegalovirus-infected cells release extracellular vesicles that carry viral surface proteins. Virology, 524, 97-105.
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2

EV and HCMV Protein Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total proteins were extracted from both EV and HCMV fractions, according to RIPA Lysis and Extraction Buffer (Thermo Fisher Scientific, Waltham, MA). 10 μg of proteins were loaded on a 4–20% precast polyacrylamide gel (Bio-Rad Laboratories, Hercules, CA) and separated by SDS-PAGE, then transferred to PVDF membranes and probed with anti-CD63 (1 μg/ml, Thermo Fisher Scientific, Waltham, MA), anti-Calnexin (1 μg/ml, Thermo Fisher Scientific, Waltham, MA), anti-Rab27A (1 μg/ml, Thermo Fisher Scientific, Waltham, MA), anti-HCMV capsid MCP (2 μg/ml), and anti-HCMV tegument pp150 (2 μg/ml) monoclonal primary anti-mouse monoclonal antibodies (clones 28–4 and 36–14) and then goat peroxidase-conjugated anti-mouse IgG secondary anti-body (Bio-Rad Laboratories, Hercules, CA). Peroxidase activity and digital images were detected by using V3 Western Workflow™ (Bio-Rad Laboratories, Hercules, CA).
Zicari S., Arakelyan A., Palomino R.A., Fitzgerald W., Vanpouille C., Lebedeva A., Schmitt A., Bomsel M., Britt W, & Margolis L. (2018). Human cytomegalovirus-infected cells release extracellular vesicles that carry viral surface proteins. Virology, 524, 97-105.
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