Log In Sign up
The largest database of trusted experimental protocols

Hnk 1

Manufactured by BioLegend
Visit Supplier
Sourced in United States

HNK-1 is a cell surface glycoprotein that is expressed on natural killer cells, some T cells, and a subpopulation of developing neurons. It functions as a neural cell adhesion molecule and is involved in cell-cell interactions during neural development.

Automatically generated - may contain errors

Lab products found in correlation

Trypsin edta, by Merck Group (2 mentions) Ssea4, by BioLegend (1 mentions) Tra 1 60, by BioLegend (1 mentions) Facscalibur, by BD (1 mentions)

Close spelling variants of this product

CD57 (HNK-1, (3 citations) CD57 (clone HNK-1), (2 citations)

2 protocols using hnk 1

1

Characterization of Differentiated Neural Crest and Corneal Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The differentiating cells derived from hPSCs were dissociated using EDTA-trypsin (Sigma) and stained with antibodies against P75 (Biolegend) and HNK-1 (Biolegend) as neural crest markers, antibodies against ATP1A1 (Biolegend) and SLC4A11 (Bioss) as corneal endothelial markers, or antibodies against the pluripotent markers TRA-1-60 (Biolegend) and SSEA4 (Biolegend) for 30 to 60 minutes on ice. Premier Data Acquisition and Analysis software (BD Biosciences) was used for analysis. Data were analyzed using FlowJo software (TreeStar).
Li Z., Duan H., Jia Y., Zhao C., Li W., Wang X., Gong Y., Dong C., Ma B., Dou S., Zhang B., Li D., Cao Y., Xie L., Zhou Q, & Shi W. (2022). Long-term corneal recovery by simultaneous delivery of hPSC-derived corneal endothelial precursors and nicotinamide. The Journal of Clinical Investigation, 132(1), e146658.
+ Open protocol
+ Expand
2

Immunophenotyping of Differentiated Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The differentiated cells were dissociated with 0.25% Trypsin-EDTA (Sigma). Briefly, 1 × 106 cells were resuspended in phosphate-buffered saline (PBS) and incubated for 30 min at 4 °C with primary conjugated antibodies P75 (Biolegend, San Diego, CA, USA) and HNK-1 (Biolegend). Then, the cells were then subjected to flow cytometry using the BD FACS Calibur and analysis was performed using the Flow-Jo program (Treestar, OR, USA).
Gong Y., Duan H., Wang X., Zhao C., Li W., Dong C., Li Z, & Zhou Q. (2021). Transplantation of human induced pluripotent stem cell-derived neural crest cells for corneal endothelial regeneration. Stem Cell Research & Therapy, 12, 214.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!