Baff human instant elisa kit

To evaluate the general indications of inflammation, CRP plasma levels were measured using a Human CRP Instant ELISA Kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer’s instructions. The samples were diluted 1:500 with assay buffer before use. Samples that were above the standard curve were further diluted and assayed a second time. BAFF measurements were performed using the BAFF Human Instant™ ELISA Kit (catalog no. BMS2007INST Thermo Fisher), according to the manufacturer’s instructions. HMGB1 plasma levels were assessed using a colorimetric Elisa Kit (Part No. NBP2-62766, Novus Biologicals, Littleton, CO, USA), according to the manufacturer’s instructions, without sample dilution. All ELISA assays were performed using a SpectraMax ABS/ABS Plus Microplate Reader (Molecular Devices, San Jose, CA, USA). Leptin plasma concentrations were measured using a Leptin Human Instant™ ELISA kit (Thermo Fisher), according to the manufacturer’s instructions. Samples were diluted 1:25 with assay buffer before use.