Caspase 3 and caspase 9 activity assay kits

Monoclonal antibodies specific of caspase-3, PAPR, caspase-9, Bcl-2, myeloid cell leukemia-1 (Mcl-1), Bax, Jun activation domain-binding protein 1 (Jab1), total STAT3 and the phosphor forms, and p21 were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Caspase-3 and caspase-9 activity assay kits were ordered from Abcam (Cambridge, MA, USA). Lipofectamine 3000 reagent was purchased from Life Technologies (AB & Invitrogen) (Carlsbad, CA, USA). STAT3 overexpression vector was obtained from OriGene Technologies, Inc. (Rockville, MD, USA). Dimethyl sulfoxide (DMSO) was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). NSCLC cells A549 were obtained from the Cell Line Bank at the Laboratory Animal Center of Sun Yat-sen University (Guangzhou, China) and PC9 and H1650 were obtained from the Chinese Academy of Sciences Cell Bank of Type Culture Collection (Shanghai, China). All cells were grown at 37°C in a humidified 5% CO₂ and 95% air and cultured in RPMI-1640 medium (Life Technologies, Carlsbad, CA, USA) containing 10% FBS (Gibco, USA) and 0.5% penicillin-streptomycin sulfate (Invitrogen Life Technologies, Carlsbad, CA, USA). Cells were counted using the automated cell counter star (Inno-Alliance Biotech Inc., Denver, CO, USA).

Monoclonal antibodies specific of Caspase-3 (cleaved Caspase-3), Caspase-9 (cleaved Caspase-9), Bcl-2, myeloid cell leukemia-1 (Mcl-1), Bax, Bim, Cytochrome C (Cyt-C), and STAT3 were purchased from Cell Signaling Technology Inc. (Beverly, MA, USA). Caspase-3 and Caspase-9 activity assay kits were ordered from Abcam (Cambridge, MA, USA). Lipofectamine 3000 reagent was purchased from Life Technologies (Carlsbad, CA, USA). SiSTAT3 (#1,2,3) and all qRT-PCR primers including Bcl-2, Mcl-1, Bax, Bim, STAT3 and GAPDH were purchased from Ribobio (Guangzhou, Guangdong, China). All primer sequences and siSTAT3 target sequences were shown in Additional file 1: Table S1. NSCLC cells A549 were obtained from the Cell Line Bank at the Laboratory Animal Center of Sun Yat-sen University (Guangzhou, China) and PC9 were obtained from the Chinese Academy of Sciences Cell Bank of Type Culture Collection (Shanghai, China). All cells were grown at 37 °C, in a humidified 5% CO₂ and 95% air and cultured in RPMI-1640 medium (Life Technologies, Carlsbad, CA, USA) containing 10% FBS (Gibco, USA) and 0.5% penicillin–streptomycin sulfate (Invitrogen, Carlsbad, CA, USA). Cells were counted using the automated cell counter star (Invitrogen, Carlsbad, CA, USA).