Apoptosis and cell cycle assay were processed with an Annexin V-Alexa Fluor 647/PI Kit (Yeasen, Shanghai, China) and Cell Cycle Analysis Kit (Yeasen, Shanghai, China), according to the manufacturer's protocol. Brie y, 10 6 cells were collected and dual-incubated with Annexin V-Alexa Fluor 647 and PI for ow cytometry assay. For cell cycle analysis, 10 5 cells per well were seeded in 6-well plate, xed in 70% ethanol overnight, and stained with PI in staining buffer for 30 min at 37℃. The procedure was protected from light, then collected for ow cytometry assay within 5 hours and analyzed using the owjo analysis software.
Annexin 5 alexa fluor 647 pi kit
Manufactured by Yeasen
Sourced in China
The Annexin V-Alexa Fluor 647/PI Kit is a laboratory reagent used for the detection and quantification of apoptotic cells. It contains Annexin V conjugated to the Alexa Fluor 647 fluorescent dye and propidium iodide (PI), which are used to identify cells undergoing early and late stages of apoptosis, respectively.
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2 protocols using annexin 5 alexa fluor 647 pi kit
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Annexin V-Alexa Fluor 647/PI Apoptosis and Cell Cycle Assay
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Annexin V-Alexa Fluor 647/PI Apoptosis and Cell Cycle Assay
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Apoptosis and cell cycle assay were processed with an Annexin V-Alexa Fluor 647/PI Kit (Yeasen, Shanghai, China) and Cell Cycle Analysis Kit (Yeasen, Shanghai, China), according to the manufacturer's protocol. Brie y, 10 6 cells were collected and dual-incubated with Annexin V-Alexa Fluor 647 and PI for ow cytometry assay. For cell cycle analysis, 10 5 cells per well were seeded in 6-well plate, xed in 70% ethanol overnight, and stained with PI in staining buffer for 30 min at 37℃. The procedure was protected from light, then collected for ow cytometry assay within 5 hours and analyzed using the owjo analysis software.
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