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2 protocols using mpeg2000

1

Nanobubble Preparation Using Lipid Extrusion

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Nanobubbles were prepared according to a previous study [13 (link),21 (link)]. Briefly, DSPC (Avanti Polar Lipids) and mPEG2000-DSPE (NOF) dissolved in methanol (DSPC: mPEG2000-DSPE = 94:6 (m/m)) were dried by evaporation and vacuum desiccated. The lipid film was dispersed in phosphate-buffered saline (PBS) at 65 °C, then sonicated for 3 min using an ultrasonic homogenizer (US 300E, Nissei, Tokyo, Japan). The liposome was enclosed with a 7.5 mL perfluoropropane gas and shaken in a sonicator (BRANSONIC B1200, Emerson Electric Co., St. Louis, MO, USA).
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2

Lipid-based Drug Delivery Formulation

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Lipids,
including egg phosphatidylcholine
(EPC) and distearylphosphatidylethanolamine-mPEG-2000 (mPEG-2000-DSPE),
were purchased from Avanti Polar Lipids. Cholesterol (CHO) was obtained
from Sigma-Aldrich. Melphalan was purchased from Dalian Meilun Biotechnology
Co., Ltd. Cell culture media and penicillin–streptomycin were
obtained from Corning Corporation (Corning). The hydrophobic fluorescent
dyes 3,3′-dioctadecyloxacarbo-cyanine perchlorate (DiO) and
1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine
iodide (DiR) were purchased from Thermo Fisher Corporation.
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