Ethical permission for animal experimentation was granted by the University of Tokyo Ethical Review Board (reference number KA16-4), and all procedures followed were in accordance with the institutional guidelines and ensured humane care of animals. The animals used were 48 female SLC/ICR mice (Japan SLC, Inc., Tokyo, Japan) of age 8–9 weeks, which were sacrificed under deep anesthesia and the popliteal and inguinal nodes were excised.
Slc icr mice
Manufactured by Japan SLC
Sourced in Japan
The Slc:ICR mice are a commonly used strain of laboratory mice. They are inbred mice that have been selectively bred to maintain specific genetic characteristics. The Slc:ICR mice are suitable for a variety of research applications.
Automatically generated - may contain errors
Lab products found in correlation
Isoflurane, by Pfizer (1 mentions)
Lab diet 5001, by Japan SLC (1 mentions)
Ain 93g mineral mixture, by Oriental Yeast (1 mentions)
Cellulose powder, by Oriental Yeast (1 mentions)
Mf diet, by Oriental Yeast (1 mentions)
L cysteine, by Fujifilm (1 mentions)
Crf 1, by Oriental Yeast (1 mentions)
Aluminum chloride hexahydrate, by Fujifilm (1 mentions)
Soybean oil, by Fujifilm (1 mentions)
Casein sodium salt, by Merck Group (1 mentions)
11 protocols using slc icr mice
1
Murine Lymph Node Extraction
2
Breeding and Husbandry of CD38 Knockout Mice
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As described previously (Akther et al. 2013 ), wild‐type male and female Slc:ICR mice (Institute of Cancer Research of the Charles River Laboratories, Inc., Wilmington, MA) were obtained from Japan SLC, Inc. (Hamamatsu, Japan) through a local distributor (Sankyo Laboratory Service Corporation, Toyama, Japan). The procedure to produce the CD38−/− mice was described previously (Kato et al. 1999 ). The offspring of wild‐type and CD38−/− mice were born in our laboratory colony. Pups were weaned at 21–28 days of age and housed in same‐sex groups of five animals until pairing. A male and female of each genotype were paired and kept in a nursing cage in our laboratory under standard conditions (24°C; 12‐h light/dark cycle, lights on at 08:00) with food and water provided ad libitum. All the animal experiments were performed in accordance with the Fundamental Guidelines for the Proper Conduct of Animal Experiments and Related Activities in Academic Research Institutions under the jurisdiction of the Ministry of Education, Culture, Sports, Science, and Technology of Japan and were approved by the Committee on Animal Experimentation of Kanazawa University.
3
Wild-type Mouse Experimental Protocol
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Eighty, six-week-old, female, wild-type Slc:ICR mice (Mus musculus musculus) obtained from Japan SLC (Shizuoka, Japan) were used throughout the experiment. All mice were
sorted into groups of three to six and acclimatized for several days in a conventional environment. Tap water and solid feed (Lab Diet 5001, Japan SLC) were supplied without restriction. At
the termination of the experiments, the mice were euthanized by exsanguination after deep anesthesia with 4% isoflurane (Pfizer Inc., New York, NY, USA). The research described herein was
approved (R03-105, R03-178) by the Animal Care and Use Committee at Tokyo University of Agriculture and Technology (TUAT), and the research was performed according to the guidelines for
animal experiments at TUAT.
sorted into groups of three to six and acclimatized for several days in a conventional environment. Tap water and solid feed (Lab Diet 5001, Japan SLC) were supplied without restriction. At
the termination of the experiments, the mice were euthanized by exsanguination after deep anesthesia with 4% isoflurane (Pfizer Inc., New York, NY, USA). The research described herein was
approved (R03-105, R03-178) by the Animal Care and Use Committee at Tokyo University of Agriculture and Technology (TUAT), and the research was performed according to the guidelines for
animal experiments at TUAT.
4
ICGN Mice Renal Development Study
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ICGN mice were bred in the National Institute of Health Sciences, Japan. Male and female ICGN/M mice showing proteinuria were mated at the age of 8 weeks old, and their 3-week-old male offspring were used in the present study. Neonatal ICGN mice were underdeveloped due to nephritic syndrome in their dams or their own renal problems. For comparison with ICR mice at the same age or with adults, 2-week-old and 7-week-old male Slc:ICR mice were purchased from Japan SLC, Inc. (Shizuoka, Japan), and infant ICR mice were maintained with their dams until being weaned. Mice were housed 5 or 6 per polycarbonate cage with sterilized softwood chips as bedding in a barrier-sustained animal room maintained at 23–25°C and 50–60% humidity with a 12 h light/dark cycle.
5
IDPN Exposure in Pregnant Mice
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IDPN (CAS , purity > 97%) was purchased from Kanto Chemical Co., Inc. (Tokyo, Japan).
Forty-eight pregnant Slc:ICR mice were purchased from Japan SLC, Inc. (Shizuoka, Japan) at gestational day (GD) 1 (the appearance of vaginal plug was designated as GD 0). Animals were individually housed with their offspring in polycarbonate cages with paper bedding until day 21 after delivery [postnatal day (PND) 21 (day of delivery is PND 0)]. Animals were kept in an experimental animal room under the condition of temperature: 23 ± 2°C, relative humidity: 55 ± 15%, 12-hr light/dark cycle. Animals were allowed access to a pelleted basal diet (MF; Oriental Yeast Co., Ltd. Tokyo, Japan) and tap water ad libitum until the start of exposure to IDPN during the experimental period. From PND 21 onwards, offspring were housed with three or four animals per cage and provided with the pellet MF basal diet and tap water ad libitum.
Forty-eight pregnant Slc:ICR mice were purchased from Japan SLC, Inc. (Shizuoka, Japan) at gestational day (GD) 1 (the appearance of vaginal plug was designated as GD 0). Animals were individually housed with their offspring in polycarbonate cages with paper bedding until day 21 after delivery [postnatal day (PND) 21 (day of delivery is PND 0)]. Animals were kept in an experimental animal room under the condition of temperature: 23 ± 2°C, relative humidity: 55 ± 15%, 12-hr light/dark cycle. Animals were allowed access to a pelleted basal diet (MF; Oriental Yeast Co., Ltd. Tokyo, Japan) and tap water ad libitum until the start of exposure to IDPN during the experimental period. From PND 21 onwards, offspring were housed with three or four animals per cage and provided with the pellet MF basal diet and tap water ad libitum.
6
ICR Mice Diet Composition Protocol
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This study was approved by the Animal Experiment Committee of Kochi Prefectural University (Approval No. 2022-7). Animal experiments were conducted in accordance with the animal experiment regulations of Kochi Prefectural University. The animal laboratory was maintained at a room temperature of 24 ± 2 °C, humidity of 55 ± 3%, and a 12-h light/dark cycle (light period: 7:00 AM to 7:00 PM, dark period: 7:00 PM to 7:00 AM). Mice introduced from the breeders were fed an MF diet (Oriental Yeast Co., Ltd., Tokyo, Japan) and drinking water (tap water) ad libitum and were pre-bred for 7 days. The experimental animals comprised 4-week-old female ICR (Slc: ICR) mice (Japan SLC, Shizuoka, Japan).
Feed ingredients included casein (FEED ONE Co., Ltd. Kanagawa, Japan), l-cysteine (FUJIFILM Wako Pure Chemical Corporation), cornstarch (Marusan, Kochi, Japan), edible oil (vitamin E-free) (Tama Biochemical Co., Ltd., Tokyo, Japan), cellulose powder (Oriental Yeast Co., Ltd., Tokyo, Japan), AIN-93G mineral mixture (Oriental Yeast Co., Ltd., Tokyo, Japan), AIN-93 vitamin with choline deuterite (Oriental Yeast Co., Ltd.), and tertiary butylhydroquinone (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan).
Feed ingredients included casein (FEED ONE Co., Ltd. Kanagawa, Japan), l-cysteine (FUJIFILM Wako Pure Chemical Corporation), cornstarch (Marusan, Kochi, Japan), edible oil (vitamin E-free) (Tama Biochemical Co., Ltd., Tokyo, Japan), cellulose powder (Oriental Yeast Co., Ltd., Tokyo, Japan), AIN-93G mineral mixture (Oriental Yeast Co., Ltd., Tokyo, Japan), AIN-93 vitamin with choline deuterite (Oriental Yeast Co., Ltd.), and tertiary butylhydroquinone (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan).
7
Developmental Toxicity of Aluminum Chloride
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Aluminum chloride hexahydrate (AlCl3·6H2O; purity: >98.0%; CAS No. 7784-13-6) was purchased from Wako Pure Chemical Industries, Ltd (Osaka, Japan). Thirty-six mated female Slc: ICR mice were purchased from Japan SLC, Inc (Hamamatsu, Japan) at gestational day (GD) 1 (appearance of vaginal plug was designated as GD 0). Maternal animals were individually housed in polycarbonate cages with paper bedding until postnatal day (PND) 21 (where PND 0 is the day of delivery). Animals were maintained in an air-conditioned animal room (temperature: 23 ± 2°C, relative humidity: 55 ± 15%) with a 12-h light/dark cycle. Maternal animals were allowed access to a pelleted basal diet (CRF-1; Oriental Yeast Co. Ltd, Tokyo, Japan) throughout the experimental period and distilled water (DW) ad libitum until the start of exposure to AlCl3. From PND 21 onwards, offspring were reared with 2 or 3 animals per cage and provided with the pellet CRF-1 basal diet and DW ad libitum.
8
Assessing Streptococcal Cellulitis in Mice
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The ability of S. pyogenes to cause cellulitis in mice after subcutaneous inoculation was assessed using a procedure described elsewhere [17 (link)]. In brief, S. pyogenes 1529, which was clinical isolates from severe invasive disease in Japan [17 (link)], was harvested after 16-hour growth on brain heart infusion agar (Eiken Chemical, Tokyo, Japan) containing 0.3% yeast extract (BHY agar) mixed in 1 mL of phosphate buffered saline (0.15 M, pH 7.2, PBS) and then centrifuged at 2,000 × g for 2 min. The pellets were diluted in 1 ml PBS to 1×108 CFU and then injected 1 × 106 CFU under the skin surface of inbred 3-week-old female Slc:ICR mice (Japan SLC, Shizuoka, Japan) using a 27-gauge needle. The number of CFU injected was verified for each experiment by plating the bacteria on BHY agar and counting CFU. The general status of mice was observed daily. In the LCEEs-treated groups, mice were gavaged with each LCEE of the fruits, leaves, or stems (1 g/kg/day) on days −1, 0, 1, and 2 after S. pyogenes inoculation, respectively. Mice in the control group were given an equal volume of PBS and were infected using the same method (Figure 1 ). The experimental procedures were conducted according to Nagoya City University Guidelines for the Care and Use of Laboratory Animals, and the study protocol was approved by the local Animal Ethics Committee of Nagoya City University (H24-M11).
9
Delamanid Efficacy in M. tuberculosis Infection
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M. tuberculosis Kurono (ATCC 35812) was used in the mouse infection model, and H37Rv (ATCC 27294) was used in the HFS-TB studies. SLC:ICR mice were obtained from Japan SLC, Inc. (Hamamatsu, Shizuoka, Japan). The hollow-fiber cartridges with polysulfone hollow fibers were purchased from FiberCell System, Inc. (New Market, MD, USA). Delamanid was supplied by Otsuka Pharmaceutical Co., Ltd. (Tokushima, Japan).
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10
Mice Welfare in Animal Research
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We purchased Slc:ICR mice from Japan SLC, Inc (Hamamatsu, Japan). All experiments were performed in accordance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals, and were approved by the Animal Research Committee of Kyoto University Graduate School of Medicine.
(MedKyo19536; Kyoto, Japan)
(MedKyo19536; Kyoto, Japan)
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