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N ε carboxyethyllysine

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N-ε-carboxyethyllysine is a chemical compound used as a standard in analytical procedures. It is a modified amino acid that can be utilized in the quantification of advanced glycation end-products.

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Lab products found in correlation

Formic acid, by Merck Group (2 mentions) N ε carboxymethyllysine, by Iris Biotech (2 mentions) O phthaldialdehyde, by Merck Group (1 mentions) Folin ciocalteu s phenol reagent, by Merck Group (1 mentions) Hplc grade methanol, by Thermo Fisher Scientific (1 mentions) Sodium acetate, by Merck Group (1 mentions) Hydrochloric acid 37 hcl, by Thermo Fisher Scientific (1 mentions) Potassium persulfate, by Thermo Fisher Scientific (1 mentions) 6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox, by Thermo Fisher Scientific (1 mentions) L serine, by Merck Group (1 mentions) Sodium azide, by Merck Group (1 mentions) O phenylenediamine, by Merck Group (1 mentions) Sodium dihydrogen phosphate dihydrate, by Merck Group (1 mentions) 2 4 6 tris 2 pyridyl s triazine, by Merck Group (1 mentions) Disodium hydrogen phosphate dihydrate, by Merck Group (1 mentions) Ethylenediaminetetracetic acid, by Merck Group (1 mentions) Acetic acid, by Merck Group (1 mentions) Go aqueous solution, by Merck Group (1 mentions) Furosine, by Iris Biotech (1 mentions) Nitrocellulose membrane, by GE Healthcare (1 mentions)

2 protocols using n ε carboxyethyllysine

1

Quantification of Advanced Glycation End-Products

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GO aqueous solution (40%), MGO aqueous solution (40%), o-phenylenediamine (OPD), o-phthaldialdehyde (OPA), ethylenediaminetetracetic acid (EDTA), gallic acid, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) diammonium salt, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), aminoguanidine (AG) hydrochloride, glucose, L-serine, sodium azide, acetic acid and formic acid were purchased from Sigma-Aldrich (St. Louis, MO). Hydrochloric acid (HCl, 37%), methanol HPLC-grade, acetonitrile HPLC- and LC-MS-grades, ammonium formate LC-MS-grade, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) and potassium persulfate were obtained from Thermo Fisher Scientific (Waltham, MA). Sodium dihydrogen phosphate dihydrate (NaH2PO4·2H2O), di-sodium hydrogen phosphate dihydrate (Na2HPO4·2H2O), sodium acetate, Folin-Ciocalteu′s phenol reagent and iron (III) chloride hexaydrate (FeCl3 6H2O) were acquired from Merck (Darmstadt, Germany). Analytical standards N-ε-carboxyethyllysine (CEL) and N-ε-carboxyethyllysine-d4 (CEL-d4), N-ε-carboxymethyllysine (CML), N-ε-carboxymethyllysine-d4 (CML-d4), methylglyoxal hydroimidazolone trifluoroacetic acid salt (MG-H1), methylglyoxal-hydroimidazolone-d3 (MG-H1-d3) and furosine were purchased from Iris Biotech GmbH (Marktredwitz, Germany).
Blidi S., Troise A.D., Zazzaroni M., De Pascale S., Cottin S., Sturrock K., Scaloni A, & Fiore A. (2024). Effect of brewer's spent grain melanoidins on maillard reaction products during storage of whey protein model systems. Current Research in Food Science, 8, 100767.
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2

Mass Spectrometry-Based Protein Analysis

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Bovine serum albumin fraction V (BSA; catalogue n. A6588), amino acids (catalogue n. LAA21), dopamine (catalogue n. H8502), acetylcholine (catalogue n. A6625) tyramine (catalogue n. T2879), γ-aminobutyric acid (GABA, catalogue n. 03,835), DL-lysine-4,4,5,5-d4 dihydrochloride (d4-lysine, catalogue n. 489,034), formic acid (catalogue n. 5.33002), salts, and buffers were purchased from Sigma-Aldrich (St. Louis, MO, USA). Nε-carboxymethyllysine (CML, catalogue n. HAA2950) and Nε-carboxyethyllysine (CEL, catalogue n. HAA2940) were obtained from Iris Biotech (Marktredwitz, Germany). Acetonitrile (catalogue n. 1.00029), water (catalogue n. 1.15333), and ammonium formate (catalogue n. 70,221) of mass spectrometry grade were obtained from Merck (Darmstadt, Germany).
Polyvinylidene difluoride (PVDF; catalogue n. GEH10600021) and nitrocellulose membranes (catalogue n GEH10600001) were from GE Healthcare (Milan, Italy).
Fuji Super RX films (catalogue n. 47,410–19,284), FujiFilm Man-X Developer (catalogue n. 949–966) and FujiFilm Man-X Fixer (catalogue n. 949–974) were from Laboratorio Elettronico Di Precisione (Naples, Italy).
Spagnuolo M.S., Mazzoli A., Nazzaro M., Troise A.D., Gatto C., Tonini C., Colardo M., Segatto M., Scaloni A., Pallottini V., Iossa S, & Cigliano L. (2022). Long-Lasting Impact of Sugar Intake on Neurotrophins and Neurotransmitters from Adolescence to Young Adulthood in Rat Frontal Cortex. Molecular Neurobiology, 60(2), 1004-1020.
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