Con-A was dissolved in phosphate-buffered saline (PBS 1×) (Sigma–Aldrich/St. Louis/MO/USA). AST, ALT, LDH, and ALP colorimetric kits were provided by Human (Wiesbaden/Germany), while γ-GT was from Spectrum Co. (Cairo, Egypt). A Caspase-3 assay kit was secured by Sigma–Aldrich (USA). MDA, NO, SOD, GSH, and TAC kits were supplied by Bio-Diagnostic Co. (Giza, Egypt). ELISA kits for myeloperoxidase (Thermo Fisher Scientific/Waltham/MA/USA), 4-HNE (4-hydroxynonenal), PC (protein carbonyl), HO-1 (heme-oxygenase-1), Bax (Bcl2 Associated X Protein), Bcl2, C-Jun N-Terminal Kinase (JNK) (MyBioSource Inc./San Diego/CA/USA), NF-ĸB p65 (Abcam/UK), TNF-α and IL-6 (R&D System/Minneapolis/MN/USA) were purchased. A TransAM Nrf2 kit (Active Motif Inc./Carlsbad/CA/USA), RNeasy MiniKit and cDNA-reverse transcription kit were also purchased (Qiagen/Germantown/MD/USA).
Nf b p65
Manufactured by Abcam
Sourced in United States
NF-κB p65 is a subunit of the NF-κB transcription factor complex. NF-κB is a key regulator of genes involved in inflammatory and immune responses.
Automatically generated - may contain errors
Lab products found in correlation
Nlrp3, by Abcam (2 mentions)
Caspase 1, by Abcam (2 mentions)
Concanavalin a (cona), by Merck Group (1 mentions)
Caspase 3 assay kit, by Merck Group (1 mentions)
Il 6 and tnf α, by R&D Systems (1 mentions)
Rneasy mini kit, by Qiagen (1 mentions)
Glutathione (gsh), by Biodiagnostic (1 mentions)
Transam nrf2 kit, by Active Motif (1 mentions)
Bioelectric amplifier, by ADInstruments (1 mentions)
Needle electrode, by ADInstruments (1 mentions)
β actin antibody, by Zhongshan Jinqiao Biotechnology (1 mentions)
Multiscango enzyme standard instrument, by Thermo Fisher Scientific (1 mentions)
Powerlab data acquisition and analysis system, by ADInstruments (1 mentions)
Txnip, by Abcam (1 mentions)
Cleaved caspase 3, by Abcam (1 mentions)
Diaminobenzidine dab, by Abcam (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Beyotime (1 mentions)
Gsdmd n, by Abcam (1 mentions)
Bca protein detection kit, by Thermo Fisher Scientific (1 mentions)
4 protocols using nf b p65
1
Hepatoprotective Effects Evaluation Protocol
2
Oxidative Stress Biomarkers and Inflammation
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The main reagents used in the present study are listed below. ROS kits were provided by Nanjings Mr Ng biological technology Co., Ltd. The CK-MB kit was provided by Wuhan huamei biological engineering Co., Ltd. IL-6, CRP, and TNF-α kits were purchased from the Wuhan optimal, born trade Co., Ltd. TXNIP, TRX, NF-ĸ Bp65, NLRP3, and Caspase-1 antibody were obtained from Abcam Trading (Shanghai) Company Ltd. β-Actin antibody was supplied by Beijing zhongshan jinqiao biotechnology Co., Ltd.
The following main instruments were used in the present study: a PowerLab signal acquisition and analysis system, a MultiscanGO enzyme standard instrument (Thermo, United States), a Sigma 3k15 high-speed refrigerated centrifuge (SIGMA, Germany), a pressure volume catheter (SPR-838, Millar company, USA), a PowerLab data acquisition and analysis system (AD Instruments, Australia), vertical electrophoresis system (BIO-TEK, USA), transfer electrophoresis system (BIO-TEK, USA), a gel imaging system (Bio Spectrum), an image analysis system (Image-Pro Plus 4.1), a PowerLab data acquisition and analysis system (AD Instruments, Australia), a bioelectric amplifier (AD Instruments, Australia), and a needle electrode (AD Instruments, Australia).
The following main instruments were used in the present study: a PowerLab signal acquisition and analysis system, a MultiscanGO enzyme standard instrument (Thermo, United States), a Sigma 3k15 high-speed refrigerated centrifuge (SIGMA, Germany), a pressure volume catheter (SPR-838, Millar company, USA), a PowerLab data acquisition and analysis system (AD Instruments, Australia), vertical electrophoresis system (BIO-TEK, USA), transfer electrophoresis system (BIO-TEK, USA), a gel imaging system (Bio Spectrum), an image analysis system (Image-Pro Plus 4.1), a PowerLab data acquisition and analysis system (AD Instruments, Australia), a bioelectric amplifier (AD Instruments, Australia), and a needle electrode (AD Instruments, Australia).
3
Quercetin's Anti-Inflammatory Effects
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Quercetin was purchased from BOC Sciences (Shirley, NY, United States). Dimethyl sulfoxide (DMSO) was purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, United States). Primary antibodies (NF-B-p65, Nrf2, HO-1, cleaved caspase-3), secondary antibody biotinylated goat anti-mouse, and diaminobenzidine DAB were purchased from Abcam Co. (Waltham, MA, United States).
4
Protein Expression Analysis of Sciatic Nerve
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The total protein of sciatic nerve tissue was extracted. First, the nucleus protein was extracted by nucleus protein extraction kit (Beyotime Bio, China). The content was detected by BCA protein detection kit (Thermo, Waltham, MA) according to the instructions. Then, the protein (30 µg/samples) with 10% SDS-PAGE was isolated and transferred to the PVDF membrane, sealed in 5% skim milk under 25°C for 1 h, and incubated with primary NF-B P65 (1:2000, Abcam Biotech, Cambridge, MA, USA), Caspase1 (1:1000, Abcam), Pro-Caspase1 (1:1000, Cusabiao, Wuhan, China), GSDMD-N (1:1000, Abcam), Klotho (1:1000, Abcam), NLRP3 (1:1000, Abcam), GAPDH (1:2500, Abcam) and H3 (1:2000, Abcam). And then, it was incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (Beyotime, Shanghai, China) for 1 h. After that, protein bands were detected with a ECL detection kit (Beyotime Biothech, Shanghai, China), GAPDH or H3 was taken as control.
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