Catalpol

Then, 6 NT samples and 6 CG samples were cut into thin slices and then dried overnight at 55 °C in blast drying oven ZRD-A5110 (Shanghai Zhicheng Analytical Instrument Manufacturing Co., Ltd., Shanghai, China). The dehydrated tuberous root samples were ground into a powder. After being filtered with a 60-mesh sieve, 1 g powder was used for HPLC determination, as described by Li et al. [21 ]. The reference substances are the following: catalpol (Mans A0215, 20 mg, Purity ≥ 98%), acteoside (Chengdu Mans A0280, 20 mg, Purity ≥ 98%) and Rhmannioside D (Chengdu Mans A1129, 20 mg, Purity ≥ 98%) from Chengdu Must Biotechnology CO.LTD, Chengdu, China.

Aspirin was purchased from by Bayer HealthCare with the batch number of J20130078). Enzyme-linked Immunosorbent Assay (ELISA) Kits including Rat interleukin 1 (IL-1), Rat S-100B protein, Rat nuclear factorκB (NF-κB), Rat neuron specific enolase (NSE), Rat high sensitive C reactive protein (HS-CRP), Rat estradiol (E2), Rat tumor necrosis factor-α (TNF-α), and Rat D-dimmer (D2D), were purchased from Nanjing Senbeijia Biological Technology Co., Ltd., Trizol was purchased from Invitrogen. Anti-AKT, Anti-PI3K, and Anti-β-actin antibody was all purchased from Abcam. All durgs (purity assay by HPLC ≥ 98%, power) including compounds of Emodin, Ginsenoside Rg1, Ginsenoside Rb1, Notoginsenoside R1, Baicalein, Geniposide, β-asarone, Taurine, Paeoniflorin, Paeonol, Apigenin, Rhein, and Catalpol, were purchased from Chengdu Must Bio-Technology Co., Ltd.