Annexin 5 kit

Manufactured by Roche

Sourced in Italy, United Kingdom

The Annexin V kit is a laboratory product used for the detection and analysis of apoptosis, a type of programmed cell death. The kit contains Annexin V, a protein that binds to phosphatidylserine, a molecule that is exposed on the surface of cells undergoing apoptosis. The kit provides a tool for researchers to study and monitor cell death processes.

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Lab products found in correlation

Tetramethylthiuram disulfide, by Merck Group (1 mentions) Poly 2 hydroxyethyl methacrylate poly hema, by Merck Group (1 mentions) A549 ccl 185, by American Type Culture Collection (1 mentions) H23 crl 5800, by American Type Culture Collection (1 mentions) Aldefluor kit, by STEMCELL (1 mentions) Fitc mouse anti human cd44, by BD (1 mentions) Cellquest, by BD (1 mentions) Facscalibur, by BD (1 mentions) Accuri c6 flow cytometer, by BD (1 mentions)

Close spelling variants of this product

Annexin V In Situ Cell Apoptosis Detection Kit Annexin V/PI Staining Kit FITC-conjugated Annexin-V/PI assay kit Annexin V-FITC-Flous staining kit Annexin-V-FLUOS Apoptosis Detection Kit Annexin V-FITC/PI Apoptosis Detection Kit Annexin V apoptosis detection kit FITC Annexin-V-FLUOS staining Kit Annexin-V-FLUOS detection kit Annexin-V-FLUOS Staining Kit

4 protocols using annexin 5 kit

Quantifying Apoptosis in Mecp2 Cells

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Apoptosis was detected with the fluorescein conjugated Annexin V Kit (Roche, Italy), following the manufacturer’s instructions. Apoptotic cells were identified with a fluorescence microscope (Leica Italia, Rome, Italy). To calculate the percentage of apoptosis in the Mecp2^+/− and CTRL MSCs, we counted, in each experiment, a minimum of 1000 cells in different fields.

Squillaro T., Alessio N., Capasso S., Di Bernardo G., Melone M.A., Peluso G, & Galderisi U. (2019). Senescence Phenomena and Metabolic Alteration in Mesenchymal Stromal Cells from a Mouse Model of Rett Syndrome. International Journal of Molecular Sciences, 20(10), 2508.

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Disulfiram and Copper Compounds in NSCLC

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The non-small cell lung cancer (NSCLC) A549 (CCL-185) and H23 (CRL-5800) cell lines were purchased from ATCC (Middlesex, UK). Copper chloride (CuCl₂, Cu), disulfiram (DS), diethyldithiocarbamate (DDC), S-methyl-N,N-diethyldithiocarbamate (S-Me-DDC), tetramethylthiuram disulfide (TMDS), 2-hydroxy-dithiobenzoic acid (HDTA), 4-imidazoledithiocarboxylic acid (IDTA), 2,4,6-Trimercaptotriazine (TMT) and poly-2-hydroxyethyl methacrylate (poly-HEMA) were purchased from Sigma (Dorset, UK). Bis(N,N-diethyldithiocarbamate)-copper(II) (Cu-DDC) was from Santa Cruz (Dallas, TX, USA). Annexin V kit was from Roche Applied Sciences (Burgess Hill, UK). Fc OxyBURST Assay Reagents was purchased from Invitrogen, Molecular Probes (Waltham, MA, USA). ALDEFLUOR kit was from StemCell Tech (Durham, NC, USA). FITC mouse Anti-Human CD44 from BD Biosciences (Oxford, UK).

Butcher K., Kannappan V., Kilari R.S., Morris M.R., McConville C., Armesilla A.L, & Wang W. (2018). Investigation of the key chemical structures involved in the anticancer activity of disulfiram in A549 non-small cell lung cancer cell line. BMC Cancer, 18, 753.

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Apoptosis Detection by Flow Cytometry

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Flow cytometric analysis of Annexin-V and propidium iodide (PI) staining which detects the exposure of phosphatidylserine and the exposed DNA content in apoptotic cells, respectively was performed using an Annexin-V kit (1-858-777, Roche). All samples were incubated with PI and Annexin-V conjugated fluorescein isothiocyante (FITC) for 15-min then analyzed by FACS Calibur (Becton Dickinson), using CellQuest (Becton Dickinson) and FlowJo software (TreeStar, Ashland, OR) for data analysis.

Chiu H.Y., Tsay Y.G, & Hung S.C. (2017). Involvement of mTOR-autophagy in the selection of primitive mesenchymal stem cells in chitosan film 3-dimensional culture. Scientific Reports, 7, 10113.

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Annexin V Apoptosis Assay for HCC1954 Cells

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Flow cytometry with Annexin V staining used to observe apoptosis. The HCC1954 cells (2.5 × 10 5 cells/mL) were seeded for 24 h and then treated with GE or Dox. The samples then incubated at 37°C and 5% CO 2 for 24 h. Next, the medium removed, and the cells were collected using trypsin. Detection of apoptosis cells was performed by staining the cells with Annexin V kit (Roche), which consisted of Annexin V and PI/RNase, and incubated for 10 min in a dark room. The cells were analyzed using BD C6 Accuri flow cytometer (BD Bioscience).

Jenie R.I., Santoso R.A., Salsabila I.A., Nugraheni N., & Meiyanto E. (2021). Alpinia galanga extract induces senescence in human epidermal growth factor receptor 2-overexpressing breast cancer cells.

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