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Anti alix sirna h

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Anti-Alix siRNA (h) is a short interfering RNA (siRNA) designed to target and suppress the expression of the Alix gene in human cells. Alix is a protein involved in various cellular processes, including endosomal trafficking and the regulation of apoptosis. This siRNA product can be used to study the role of Alix in cellular function and dynamics.

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Lab products found in correlation

Basic nucleofector kit, by Lonza (2 mentions) Anti occludin 27 mer sirna h, by OriGene (2 mentions) Anti caveolin 1 27 mer sirna h, by OriGene (2 mentions)

Close spelling variants of this product

SiRNAs (95 citations) Anti-Alix (56 citations)

2 protocols using anti alix sirna h

1

Silencing of Cav-1, Occludin, and Alix

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Cav‐1, ocln, and Alix silencing was performance using the following small interfering RNA (siRNA): anti‐occludin 27‐mer siRNA (h): Cat# SR321170, anti‐caveolin‐1 27‐mer siRNA (h): Cat# SR319567 (OriGene, Rockville, MD, USA), and anti‐Alix siRNA (h): Cat# sc‐60149 (Santa Cruz Biotechnology, Dallas, TX, USA). Trilencer‐27 universal scrambled (SCR) silencer negative control siRNA Cat# SR30004 (OriGene) was used as nonspecific control siRNA. Pericytes were transfected by Amaxa Nucleofector Technology with 1 µg siRNA or control siRNA per 106 cells using the Basic Nucleofector Kit originally designed for primary mammalian endothelial cells (Lonza, Switzerland, EU, Cat# VPI‐1001). Next day, cells were washed and allowed to recover in normal pericyte medium. For ocln overexpression experiments, ocln cDNA cloned into the pCMV6 plasmid was obtained from OriGene (Cat# RC206468). As a negative control, the pCMV6 plasmid (OriGene Cat# PS100001) was used. Cells were transfected with 2 µg of ocln overexpressing vector per 106 cells or negative control vector by using Amaxa Nucleofector Technology.
Torices S., Roberts S.A., Park M., Malhotra A, & Toborek M. (2020). Occludin, caveolin‐1, and Alix form a multi‐protein complex and regulate HIV‐1 infection of brain pericytes. The FASEB Journal, 34(12), 16319-16332.
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2

Silencing and Overexpressing Junctional Proteins

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Cav-1, ocln, and Alix silencing was performance using the following small interfering RNA (siRNA): anti-occludin 27-mer siRNA (h): Cat# SR321170, anti-caveolin-1 27-mer siRNA (h): Cat# SR319567 (OriGene, Rockville, MD, USA), and anti-Alix siRNA (h): Cat# sc-60149 (Santa Cruz Biotechnology, Dallas, TX, USA). Trilencer-27 universal scrambled (SCR) silencer negative control siRNA Cat# SR30004 (OriGene) was used as non-specific control siRNA. Pericytes were transfected by Amaxa Nucleofector Technology with 1 μg siRNA or control siRNA per 106 cells using the Basic Nucleofector Kit originally designed for primary mammalian endothelial cells (Lonza, Switzerland, EU, Cat# VPI-1001). Next day, cells were washed and allowed to recover in normal pericyte medium. For ocln overexpression experiments, ocln cDNA cloned into the pCMV6 plasmid was obtained from OriGene (Cat# RC206468). As a negative control, the pCMV6 plasmid (OriGene Cat# PS100001) was used. Cells were transfected with 2 μg of ocln overexpressing vector per 106 cells or negative control vector by using Amaxa Nucleofector Technology.
Torices S., Roberts S.A., Park M., Malhotra A, & Toborek M. (2020). Occludin, caveolin‐1, and Alix form a multi‐protein complex and regulate HIV‐1 infection of brain pericytes. The FASEB Journal, 34(12), 16319-16332.
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