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Anti gapdh hrp conjugated monoclonal antibody

Manufactured by Santa Cruz Biotechnology
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The Anti-GAPDH HRP conjugated monoclonal antibody is a reagent used for the detection of the GAPDH protein in various applications. It is a conjugate of a monoclonal antibody specific to GAPDH and the enzyme horseradish peroxidase (HRP). This antibody can be used in techniques such as Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assays (ELISA) to identify and quantify the GAPDH protein in biological samples.

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Lab products found in correlation

Polyvinylidene diflouride membrane, by Merck Group (2 mentions) Anti human cdk2 rabbit monoclonal, by Cell Signaling Technology (2 mentions) Anti human cdk4 mouse monoclonal, by Cell Signaling Technology (2 mentions) Anti human c myc rabbit monoclonal d84c12, by Cell Signaling Technology (2 mentions) Anti human p53 mouse monoclonal clone 1c12, by Cell Signaling Technology (2 mentions) Anti human max mouse monoclonal, by Abcam (2 mentions) Anti beta actin hrp conjugated monoclonal, by Santa Cruz Biotechnology (2 mentions) Hrp conjugated goat anti rabbit or anti mouse secondary antibodies, by Santa Cruz Biotechnology (2 mentions)

Close spelling variants of this product

Anti-GAPDH monoclonal antibody HRP-conjugated GAPDH GAPDH monoclonal antibody GAPDH-HRP antibody Anti-GAPDH antibody GAPDH-HRP GAPDH antibody Anti-GAPDH GAPDH

2 protocols using anti gapdh hrp conjugated monoclonal antibody

1

Western Blot Analysis of Cell Signaling Proteins

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Western blottng was performed as previously described. Protein was transferred to polyvinylidene diflouride membranes (Millipore) and immunoblotted with anti-human CDK2 rabbit monoclonal, anti-human CDK4 mouse monoclonal, anti-human c-Myc rabbit monoclonal (D84C12), or anti-human p53 mouse monoclonal (clone:1C12) antibodies (all at 1:2000; Cell Signaling). Additional antibodies included anti-human MAX mouse monoclonal (1:2000; Abcam), anti-beta Actin HRP conjugated monoclonal (1:5000; Santa Cruz), or anti-GAPDH HRP conjugated monoclonal antibody (1:2000; Santa Cruz) for at least 1h at room temperature or 4°C overnight. HRP–conjugated goat anti-mouse or anti-rabbit secondary antibodies (Santa Cruz) were added when needed.
Lu Y.C., Sanada C., Xavier-Ferrucio J., Wang L., Zhang P.X., Leighton Grimes H., Venkatasubramanian M., Chetal K., Aronow B., Salomonis N, & Krause D.S. (2018). The Molecular Signature of Megakaryocyte-Erythroid Progenitors Reveals a Role for the Cell Cycle in Fate Specification. Cell reports, 25(8), 2083-2093.e4.
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2

Western Blot Analysis of Cell Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blottng was performed as previously described. Protein was transferred to polyvinylidene diflouride membranes (Millipore) and immunoblotted with anti-human CDK2 rabbit monoclonal, anti-human CDK4 mouse monoclonal, anti-human c-Myc rabbit monoclonal (D84C12), or anti-human p53 mouse monoclonal (clone:1C12) antibodies (all at 1:2000; Cell Signaling). Additional antibodies included anti-human MAX mouse monoclonal (1:2000; Abcam), anti-beta Actin HRP conjugated monoclonal (1:5000; Santa Cruz), or anti-GAPDH HRP conjugated monoclonal antibody (1:2000; Santa Cruz) for at least 1h at room temperature or 4°C overnight. HRP–conjugated goat anti-mouse or anti-rabbit secondary antibodies (Santa Cruz) were added when needed.
Lu Y.C., Sanada C., Xavier-Ferrucio J., Wang L., Zhang P.X., Leighton Grimes H., Venkatasubramanian M., Chetal K., Aronow B., Salomonis N, & Krause D.S. (2018). The Molecular Signature of Megakaryocyte-Erythroid Progenitors Reveals a Role for the Cell Cycle in Fate Specification. Cell reports, 25(8), 2083-2093.e4.
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