In addition, the employed Mycobacterium tuberculosis ATCC 27294 (Mtb) was grown in 7H9-medium containing 7H9 BBL Middlebrook broth (BD), glycerol (Honeywell, Charlotte, NC, USA) OADC (Oelic Albumin Dextrose Catalase; BD), Tween 80 (Roth, Karlsruhe, Germany), and ddH2O. The pH was adjusted between 7.2–7.4 and sterile filtration was performed with a 0.2-µm filter membrane (Thermo ScientificTM NalgeneTM Rapid-FlowTM, Thermo Scientific, Bremen, Germany).
Bbl middlebrook broth
BBL Middlebrook broth is a culture medium used for the cultivation and identification of Mycobacterium species, such as Mycobacterium tuberculosis. It provides the necessary nutrients and growth factors for the cultivation of these bacteria.
Lab products found in correlation
2 protocols using bbl middlebrook broth
Cultivation of Antibiotic-Resistant Bacteria
In addition, the employed Mycobacterium tuberculosis ATCC 27294 (Mtb) was grown in 7H9-medium containing 7H9 BBL Middlebrook broth (BD), glycerol (Honeywell, Charlotte, NC, USA) OADC (Oelic Albumin Dextrose Catalase; BD), Tween 80 (Roth, Karlsruhe, Germany), and ddH2O. The pH was adjusted between 7.2–7.4 and sterile filtration was performed with a 0.2-µm filter membrane (Thermo ScientificTM NalgeneTM Rapid-FlowTM, Thermo Scientific, Bremen, Germany).
Antimicrobial Activity Evaluation of AMPs
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