Mouse il 2 elisa kit

The human serum levels of TNF-α and IL-6 were measured via Human IL-6 ELISA Kit (EK0410) and Human TNFα ELISA Kit (EK0525), and the mouse serum levels of IL-2, TNF-α, hs-CRP, IFN-γ, IL-4, IL-17A, and IL-10 were determined by Mouse IL-2 ELISA Kit (EK0398), Mouse TNFα ELISA Kit (EK0527), Mouse IFN gamma/IFNG ELISA Kit (EK0375), Mouse IL-4 ELISA Kit (EK0405), Mouse IL-17A ELISA Kit (EK0431), and Mouse IL-10 ELISA Kit (EK0417, BOSTER, Wuhan, China) in line with the manufacturer's protocol. The mouse serum levels of hs-CRP were analyzed through Mouse C Reactive Protein ELISA Kit (ab222511, Abcam, Cambridge, UK) based on the working instructions. The absorbance at 450 nm was measured using a microplate reader (SpectraMAX Plus384, America).

Konjac glucomannan was purchased from Sanyuan Tianyu biological products co. LTD (Xian, China). KGM which is a kind of natural neutral polysaccharide is mainly composed of D‐glucose (G) and D‐mannose (M) monomers in M/G molar ratio of 1.5–1.6 connecting by β‐1,4 glycosidic bonds with about 1 acetyl group in every 17–19 sugar units at the C‐6 position. Fetal bovine serum (FBS), Dulbecco's modified Eagle's medium (DMEM), and RPMI‐1640 were purchased from GIBCO BRL (Carlsbad, CA, USA). CTX, 3‐(4, 5‐dimethyl‐thiazol‐2‐yl)‐2, 5‐diphenyl‐tetrazolium bromide (MTT), and levamisole (LMS) were bought from Sigma‐Aldrich (St. Louis, USA). Concanavalin‐A, Mouse TNF‐α ELISA kit, Mouse IL‐2 ELISA kit, and Mouse IgG ELISA kit were provided by Boster Biological Technology (Wuhan, China). YAC‐1 cells, sheep red blood cells (SRBC), neutral red dye, trypan blue, and complement were obtained from Beijing Suolaibao Biological Engineering co. Ltd (Beijing, China). Penicillin, pancreatic enzymes, and Cell Counting Kit‐8 (CCK‐8) were bought from Shanghai Yisheng Biotechnology co., Ltd (Shanghai, China).