In glioma cells, the indicated plasmids were transfected. Collect the trypsin-digested glioma cells 72 hours later in a 15 mL centrifuge tube, centrifuge (800 rpm, 5 minutes), and discard the supernatant. Glioma cells were extracted using extraction buffer and ultrasonic cell disruption (power 20%, sonication 3 seconds, interval 10 seconds, repeated 30 times), followed by 10 minutes of sharking in a 100°C water bath and centrifugation at 10000g at room temperature. The proline level in the supernatant was determined using the Proline Content Assay Kit (Nanjing Jiancheng Bioengineering Institute, BCO295, 100T/96S), the absorbance of the sample was measured at 520 nm using an enzyme calibrator, and a standard curve was developed based on the concentration of the standard to determine the proline content.
Proline content assay kit
Manufactured by Nanjing Jiancheng
Sourced in China
The Proline Content Assay Kit is a laboratory tool designed to quantify the proline content in various samples. It provides a reliable and standardized method to measure proline levels, which is useful for applications in fields such as plant biology, microbiology, and biochemistry.
Automatically generated - may contain errors
2 protocols using proline content assay kit
1
Proline Content Quantification in Glioma Cells
2
Oxidative Stress Response in Arabidopsis spl36 Mutant
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At the heading stage, 10 randomly selected WT and spl36 leaves of obvious phenotype and similar growth were stained with trypan blue and DAB solution according to the method of Dietrich et al. (1994) (link) and Thordal-Christensen et al. (1997) . Meanwhile, the first, second, and third leaves of the WT and spl36 mutant were collected and used to measure ROS and proline content, as well as catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) activity as previously described. The Superoxide Anion Assay Kit (A052), Proline Content Assay Kit (A107), SOD Activity Assay Kit (A001-1), CAT Assay Kit (A007-1), and POD Activity Assay Kit (A084-3) were provided by the Nanjing Jiancheng Technology Company (Nanjing, China).
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