Facsaria flow cytometer

Manufactured by BD

Sourced in United States, Germany, Canada, Sweden, United Kingdom, Japan, Spain

The BD FACSAria flow cytometer is a high-performance cell sorting instrument designed for advanced research applications. It utilizes fluorescence-activated cell sorting technology to accurately identify and separate individual cells from complex samples based on their unique physical and biochemical characteristics.

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Lab products found in correlation

Facsdiva software, by BD (35 mentions) Propidium iodide, by Merck Group (17 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (14 mentions) Cellquest software, by BD (9 mentions) Facscalibur, by BD (8 mentions) Aldefluor kit, by STEMCELL (8 mentions) Facsdiva, by BD (8 mentions) Facsdiva 6, by BD (7 mentions) Fitc annexin 5 apoptosis detection kit, by BD (7 mentions) Lsrii flow cytometer, by BD (7 mentions) Taqman assay primer set, by Thermo Fisher Scientific (6 mentions) Dnase 1, by Merck Group (6 mentions) 4 6 diamidino 2 phenylindole (dapi), by BD (6 mentions) Cytofix cytoperm kit, by BD (6 mentions) Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (5 mentions) Propidium iodide, by BD (5 mentions) Zombie aqua fixable viability kit, by BioLegend (5 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions) Fluorescence minus one controls, by BD (5 mentions) Lsrii instrument, by BD (5 mentions)

Close spelling variants of this product

FACSAria II flow cytometer (701 citations) BD FACSAria IIu flow cytometer (22 citations) BD FACSAria II SORP Flow Cytometer Cell Sorter (4 citations) BD FACSAriaTM II cell sorter flow cytometer (2 citations) BD FACSAria II SORP flow cytometer (16 citations) FacsAria Illu Flow Cytometer (4 citations) FACSAria II cell flow cytometer (2 citations) BD FACSAria TM Fusion II flow cytometer (2 citations) FACSAria Special Order Research Product flow cytometer/sorter (3 citations) FACSAria Flow Cytometer Cell Sorter (4 citations)

761 protocols using facsaria flow cytometer

Cellular Redox and Mitochondrial Membrane Potential Assessment with ZnO Nanoparticles

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The cellular redox state was measured by oxidation of 2′,7′-dichlorodihydrofluorescein diacetate (H₂DCFDA) into 2,7-dichlorodihydrofluorescein (DCF) which is a redox indicator probe [48 (link)]. H9c2 cells (200 × 10³) were cultured with ZnO NPs (5, 10, 20 μg/cm²) for 1, 3, and 6 h in 56-mm glass Petri dishes. After treatment, cells were trypsinized and incubated with H₂DCFDA (10 μM) for 30 min, and they were washed twice with PBS. Fluorescence was quantified in a FACSAria flow cytometer (Becton-Dickinson, CA, USA).
Oxidative stress was also tested through ΔΨm changes based on the fluorescent dye rhodamine 123 (Rh123). For this, cell suspensions (1 × 10⁶) were treated with ZnO NPs (5, 10, 20 μg/cm²) for 1 h. Then, cells were washed with PBS and incubated with Rh123 (5 μg/mL) for 15 min. After incubation, cells were washed with PBS and analyzed in a FACSAria flow cytometer (Becton-Dickinson, CA, USA). Data was processed using FlowJo 8.7 software (Stanford University).

Mendoza-Milla C., Macías Macías F.I., Velázquez Delgado K.A., Herrera Rodríguez M.A., Colín-Val Z., Ramos-Godinez M.D., Cano-Martínez A., Vega-Miranda A., Robledo-Cadena D.X., Delgado-Buenrostro N.L., Chirino Y.I., Flores-Flores J.O, & López-Marure R. (2022). Zinc Oxide Nanoparticles Induce Toxicity in H9c2 Rat Cardiomyoblasts. International Journal of Molecular Sciences, 23(21), 12940.

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Annexin V-FITC Apoptosis Assay for Cell Lines

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The most effective antiproliferative agents
in this series (4f, 4h, 4i, 4k, and 4l) were incubated for 24 h at IC₅₀ concentrations on A549 and L929 cells. Phosphatidylserine
externalization, a marker of early apoptosis, was assessed using the
FITC Annexin V apoptosis detection kit (BD Pharmingen, San Jose, CA,
USA) on a BD FACSAria flow cytometer. Following their collection,
the A549 and L929 cells were twice washed in ice-cold PBS before being
resuspended in 100 μL of binding buffer. The cells were treated
with a volume of 5 μL (5 μg/mL) of Annexin V-FITC and
PI, and they were incubated for 15 min at room temperature (20–25
°C) in the dark. Following that, 400 μL of binding buffer
was added to the combination samples, and FACSDiva version 6.1.1 was
used to analyze the samples on a BD FACSAria flow cytometer.

Yurttaş L., Evren A.E., Kubilay A., Aksoy M.O., Temel H.E, & Akalın Çiftçi G. (2023). Synthesis of Some New 1,3,4-Oxadiazole Derivatives and Evaluation of Their Anticancer Activity. ACS Omega, 8(51), 49311-49326.

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Isolation and Co-culture of Immune Cells

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Human cells were acquired from patients with ESCC for FACS. CD45⁺CD3⁺CD4⁺ T cells and CD45⁺CD3⁺CD8⁺ T cells were sorted on a BD FACsAria flow cytometer based on CD45, CD3, CD4, and CD8 surface expression phenotypes. CD45⁺CD19⁺CD55⁺ B cells and CD45⁺CD19⁺CD55⁻ B cells were sorted on the BD FACsAria flow cytometer based on CD45⁺, CD19⁺, and CD55^+/− surface expression phenotypes. Purified B-cell (2 × 10⁴ cells/well) and T-cell (2 × 10⁵ cells/well) subsets from the same patient were plated in a 48-well U-bottom plate and co-cultured for 48 h with anti-CD3 (2 mg/mL), anti-CD28 (10 mg/mL), interleukin (IL)-2 (0.2 mg/mL), anti-IgM (2 mg/mL), and lipopolysaccharide (10 mg/mL). Flow cytometry was used to analyse the composition of T cells.

Zhang H., Wen H., Zhu Q., Zhang Y., Xu F., Ma T., Guo Y., Lu C., Zhao X., Ji Y., Wang Z., Chu Y., Ge D., Gu J, & Liu R. (2024). Genomic profiling and associated B cell lineages delineate the efficacy of neoadjuvant anti-PD-1-based therapy in oesophageal squamous cell carcinoma. eBioMedicine, 100, 104971.

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Enrichment and Sorting of CD45+ Semen Cells

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Seminal cells were separated from seminal plasma immediately after collection by centrifugation for 15 min at 775 x g, resuspended in 1 ml cold PBS supplemented with 10% FCS and 2 mM EDTA, and kept on ice for no more than 1 h. Cells were then centrifuged for 10 min at 1500 x g, filtered through a 70-µM sieve, and washed with 5 ml cold PBS supplemented with 10% FCS and 2 mM EDTA.
CD45⁺ semen cells from animals #BA865F, #1103075, #CA147F, and #MF1414 were enriched using CD45 magnetic beads and cell sorting with a BD FACS Aria Flow Cytometer. Total semen cells were incubated for 15 min at 4 °C with 20 μl anti-CD45 magnetic beads (Miltenyi Biotec) and washed once with 2 ml cold PBS supplemented with 0.5% BSA and 2 mM EDTA (sorting buffer). The CD45⁺ cell fraction was then enriched by magnetic-bead sorting, using LS columns (Miltenyi Biotec), according to the manufacturer's instructions. Cells were eluted in 4 ml sorting buffer. Following the magnetic bead-based enrichment process, cells were stained with amine-reactive blue dye (Life Technologies) to identify the dead cells, and anti-CD45 (clone D058-1283, BD Pharmingen). Cells were washed twice and stored at 4 °C in PBS/10% FCS. CD45⁺ cells were then sorted by simultaneous four-way sorting on a FACS Aria flow cytometer (BD Biosciences). The enriched cell fraction was used in the neutralization assay.

Suphaphiphat K., Tolazzi M., Hua S., Desjardins D., Lorin V., Dereuddre-Bosquet N., Mouquet H., Scarlatti G., Grand R.L, & Cavarelli M. (2020). Broadly neutralizing antibodies potently inhibit cell-to-cell transmission of semen leukocyte-derived SHIV162P3. EBioMedicine, 57, 102842.

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Measuring Apoptosis by Annexin V Staining

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After the cells were incubated with the most potent antiproliferative agents in this series at IC₅₀ concentrations, phosphatidylserine externalization, which indicates early apoptosis, was measured by the FITC Annexin V apoptosis detection kit (BD Pharmingen, San Jose, CA, USA) on a BD FACSAria flow cytometer for 24 h. Annexin V staining protocol was applied according to the manufacturer’s instructions (BD Pharmingen, San Jose, CA, USA). The cells were then briefly washed with cold phosphate buffer saline (PBS) and suspended in a binding buffer at a concentration of 1 × 10⁶ cells/mL. Then, 100 µL of this solution containing 1 × 10⁵ cells was transferred to a 5 mL test tube. After 5 µL of Annexin V and PI was added, the cells were incubated for 15 min at room temperature in the dark. Then 400 µL of 1× binding buffer was added to each tube and the cells were processed for data acquisition, and analyzed on a BD FACSAria flow cytometer using FACSDiva version 6.1.1 software (BD Biosciences, San Jose, CA, USA).

Altıntop M.D., Sever B., Akalın Çiftçi G, & Özdemir A. (2018). Design, Synthesis, and Evaluation of a New Series of Thiazole-Based Anticancer Agents as Potent Akt Inhibitors. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(6), 1318.

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Regulatory T Cell Suppression Assay

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Tresp (CD4+CD25-) and Tregs (CD4+CD25+CD127 dim ) from PBMCs were cultured at a 1:2 Treg to Tresp ratio. Cell cultures were incubate with isotype control antibody or CD152(CTLA-4)-F(ab')2 (10μg /ml) plus PPD (25μg /ml). After three days of incubation in RPMI medium supplemented with 10% (vol/vol) FBS, cells were stained with antibodies and analyzed by BD FACS Aria flow cytometer, and harvested with TRIZol for real-time PCR detection as described above. For the CFSE-labeled T (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted May 13, 2020. ; https://doi.org/10.1101/2020.05.11.089946 doi: bioRxiv preprint 7 lymphocyte proliferation inhibition assay, cells were incubated for 7 days and at 3 rd day plus IL-2, and at the 7 th day, cells were collected and stained with antibodies and analyzed by BD FACS Aria flow cytometer.

Shao L., Gao Y., Shao X., Ou Q., Shu Z., Liu Q., Zhang B., Wu J., Ruan Q., Shen L., Weng X., Zhang W., & Chen Z.W. (2020). CTLA-4 blockade reverses the Foxp3+ T-regulatory-cell suppression of anti-tuberculosis T-cell effector responses.

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Quantification of Cell Surface Sialic Acids

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A549 cells were transfected with FFAR2 siRNA or scrambled siRNA (30 nM). After 48 h, the cells were trypsinized, fixed with 4% PFA for 20 min, and then stained with an Alexa Fluor 488 conjugate of wheat germ agglutinin (WGA) (Invitrogen), a commonly used agent for the detection of sialic acids (SAs) on the cell surface. After two washes with PBS, the cell suspensions were subjected to flow cytometry on a FACSAria flow cytometer (BD Biosciences, Franklin Lakes, NJ). In a separate experiment, A549 cells treated with siRNA and fixed with PFA were stained with biotinylated Maackia amurensis lectin (MAL) (α-2,3-SA) or Sambucus nigra lectin (SNA) (α-2,6-SA) (Vector Laboratories, Burlingame, CA). The bound biotinylated lectins were captured by streptavidin conjugated with Cy-5 (Life Technologies). After three washes, the cell suspensions were subjected to flow cytometry on a FACSAria flow cytometer (BD Biosciences). A549 cells pretreated with neuraminidase (Sigma-Aldrich) at 37°C for 2 h were stained with WGA, MAL, or SNA and used as negative controls. The data were analyzed by using FlowJo software (FlowJo, Ashland, OR).

Wang G., Jiang L., Wang J., Zhang J., Kong F., Li Q., Yan Y., Huang S., Zhao Y., Liang L., Li J., Sun N., Hu Y., Shi W., Deng G., Chen P., Liu L., Zeng X., Tian G., Bu Z., Chen H, & Li C. (2020). The G Protein-Coupled Receptor FFAR2 Promotes Internalization during Influenza A Virus Entry. Journal of Virology, 94(2), e01707-19.

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Apoptosis and Cell Cycle Analysis

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HFLS-RA cells were transfected and harvested after 48 h, and processed as per the PE Annexin V Apoptosis Detection Kit (BD, USA). Briefly, 5 μl of the PE reagent and 5 μl of 7-AAD reagent were added into the cell suspensions and incubated for 15 min at room temperature. Finally, we analyzed the cells in different phases using the FACSAria flow cytometer (BD).
HFLS-RA cells were harvested 48 h after transfection and fixed with 75% ethanol at −20°C for at least 2 h. Subsequently, cells were resuspended in the PI reagent (BD). Finally, we analyzed the cells in different phases using the FACSAria flow cytometer (BD).

Wang Y., Hou L., Yuan X., Xu N., Zhao S., Yang L, & Zhang N. (2020). LncRNA NEAT1 Targets Fibroblast-Like Synoviocytes in Rheumatoid Arthritis via the miR-410-3p/YY1 Axis. Frontiers in Immunology, 11, 1975.

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Cell Apoptosis and Cell Cycle Analysis

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Cells (2 × 10⁵ cells/well) were seeded into 6-well plates and cultured for 24 h. The compounds (NC, GSK343, DZNep, gefitinib, G + g, and D + g) were added at various indicated concentrations for 48 h. For the cell apoptosis assay, cells were stained using the Annexin V-FITC Apoptosis Analysis Kit (BD Biosciences, San Jose, CA, USA) and were analyzed using the FACSAria™ flow cytometer (BD Biosciences). For the cell cycle assay, cells were trypsinized and fixed with 70% ice-cold ethanol overnight. Subsequently, cells were treated with DNase-free ribonuclease (TaKaRa, Beijing, China), stained with propidium iodide (PI; BD Biosciences), and analyzed using the FACSAria™ flow cytometer (BD Biosciences) equipped with ModFit LT (Topsham, ME, USA).

Gong H., Li Y., Yuan Y., Li W., Zhang H., Zhang Z., Shi R., Liu M., Liu C., Chen C., Liu H, & Chen J. (2020). EZH2 inhibitors reverse resistance to gefitinib in primary EGFR wild-type lung cancer cells. BMC Cancer, 20, 1189.

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Cell Cycle and Apoptosis Analysis of Drug Treatments

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Following treatment with different drugs for 24 h, the cells were harvested and fixed in 70% ice-cold ethanol at 4°C for 30 min. After washing with PBS, the cells were incubated with propidium iodide staining buffer (BD Pharmingen, San Diego, CA, USA) for 15 min and analyzed by FACSAria flow cytometer. The experiment was performed in triplicate.
For apoptosis analysis, the cells were treated for 24 h with XAV939 (8 μmol/l), 5-FU (50 μmol/l), XAV939 (8 μmol/l) + 5-FU (50 μmol/l), DDP (8 μmol/l) and XAV939 (8 μmol/l) + DDP (8 μmol/l), separately. The cells were measured using FACSAria flow cytometer (BD Biosciences, USA), and Annexin V (+) cells were counted for apoptotic cells after Annexin V-fluorescein isothiocyanate/propidium iodide (FITC/PI) (BD Pharmingen) double staining. The experiment was performed in triplicate.

WU X., LUO F., LI J., ZHONG X, & LIU K. (2016). Tankyrase 1 inhibitior XAV939 increases chemosensitivity in colon cancer cell lines via inhibition of the Wnt signaling pathway. International Journal of Oncology, 48(4), 1333-1340.

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