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Vglut2 ires cre knock in mice

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Vglut2-ires-Cre knock-in mice are a genetically engineered mouse model that expresses Cre recombinase under the control of the Vglut2 (Vesicular Glutamate Transporter 2) gene promoter. This allows for the selective targeting and manipulation of neurons that express Vglut2, which is a marker for glutamatergic neurons.

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Lab products found in correlation

Ai3 mice, by Jackson ImmunoResearch (1 mentions) C57bl 6j, by Charles River Laboratories (1 mentions) Thy1 yfp mice, by Jackson ImmunoResearch (1 mentions) Vglut2 ires cre, by Jackson ImmunoResearch (1 mentions) Vgat ires cre, by Jackson ImmunoResearch (1 mentions) Wild type c57bl 6j mice, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

Vglut2-ires-Cre (61 citations) Vglut2-Cre (33 citations) Vglut2-ires-Cre mice (16 citations) Vglut2-Cre mice (14 citations) VGlut2-ires-cre knock-in (3 citations) VGLUT2 (3 citations) Vglut2-cre knock-in (2 citations)

4 protocols using vglut2 ires cre knock in mice

1

Transgenic Mouse Models for VGluT2 Neuron Studies

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All surgical and experimental protocols were approved by the Animal Use and Care Committee of Hubei University of Medicine (Shiyan, China) and were performed in accordance with the National Institutes of Health guidelines. VGluT2-IRES-Cre knock-in mice (stock #028863) and Ai3 mice (stock #007903) were obtained from The Jackson Laboratory (Bar Harbor, ME, United States). Wild-type C57BL/6 (WT) mice were provided by the Institute of Laboratory Animal Science, Hubei University of Medicine. The VGluT2::Ai3 mice were generated by crossing male Vglut2-cre+/+ mice with female Ai3 mice. The Vglut2-cre+/– mice were generated by crossing male Vglut2-cre+/+ mice with female WT mice. The mice were kept in a 12:12 (06:00–18:00) light: dark cycle and were given free access to food and water. Unless otherwise stated, 8–12-week-old male and female mice were used for all experiments. All the studies and tests were conducted between 9 am and 6 pm.
Ouyang J., Chen X., Su S., Li X., Xu X., Yu X., Ke C, & Zhu X. (2021). Neuroligin1 Contributes to Neuropathic Pain by Promoting Phosphorylation of Cofilin in Excitatory Neurons. Frontiers in Molecular Neuroscience, 14, 640533.
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2

Fluorescent Mouse Strains for Neuroscience

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The datasets mScarlet in PAG, YFP in CTX, and GFAP in HC were acquired for this study. Here, all mice were bred in the animal facility of the Institute of Clinical Neurobiology at the University Hospital of Würzburg, Germany, and housed under standard conditions (55 ± 5% humidity, 21 ± 1 C, 12:12-h light:dark cycle) with access to food and water ad libitum. VGlut2-IRES-Cre knock-in mice65 (link) (stock no. 208863), as well as Thy1-YFP mice63 (link) (stock no. 003782), were obtained from Jackson Laboratory. Additionally, we used wild-type mice with the genetic background C57BL/6J (Charles River, CRL:027). Only male mice at ages between 4 and 8 months were used.
Griebel M., Segebarth D., Stein N., Schukraft N., Tovote P., Blum R, & Flath C.M. (2023). Deep learning-enabled segmentation of ambiguous bioimages with deepflash2. Nature Communications, 14, 1679.
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3

Transgenic Mouse Models for Neuroscience

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All procedures were approved by the IACUC of NYULMC in compliance with the NIH guidelines for the care and use of laboratory animals. Mice were housed under a 12 h light-dark cycle (12 p.m. to 12 a.m. light), with food and water available ad libitum. Test animals were adult Esr1-2A-Cre female mice (> 10 weeks). They were originally provided by D.J. Anderson (Lee et al., 2014 (link)) and now available from Jackson Laboratory (Stock No. 017911). Vgat-ires-Cre and Vglut2-ires-Cre knock-in mice (Vong et al., 2011) were provided by B. Lowell and are now from Jackson Laboratory (stock No: 016962 and 016963). DAT-ires-Cre line was purchased from Jackson laboratory (stock No.006660). Ai6 (Madisen et al., 2010) was purchased from the Jackson Laboratory (Stock No.007906) and crossed with Vgat-ires-Cre and Vglut2-ires-Cre mice. Stimulus animals were 3–10 days old pups from C57BL/6N pairs. For all functional manipulation experiments, the age matched pups were used for control and test groups. After surgery, all the animals are single housed or housed with the litter. All the experiments are performed during the dark cycle of the animals.
Fang Y.Y., Yamaguchi T., Song S.C., Tritsch N.X, & Lin D. (2018). A hypothalamic-midbrain pathway essential for driving maternal behaviors. Neuron, 98(1), 192-207.e10.
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Protocol for Transgenic Mouse Experiments

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CRH-ires-Cre mice were generated previously21 (link). C57BL/6J wild type mice, Emx1-ires-Cre
and Vglut2-ires-Cre knock-in mice, and Rosa-floxstop-GFP reporter mice were
purchased from the Jackson Laboratory. All procedures involving mice were
approved by the Fred Hutchinson Cancer Research Center Institutional Animal Care
and Use Committee.
We used both males and females in all experiments, with similar numbers
where possible. The same number of each sex was used in experiments in Fig. 1 and Extended Data Figs 2, 3, 5 and 6.
No statistical methods were used to predetermine sample size. Animals
were randomly chosen for experimental subjects. Animals were excluded from
certain experiments. For Arc experiments in Fig.
2, animals were excluded if PRV+ cells were not found in all
5 OC and 3 VA areas analyzed. For chemogenetic activation or silencing of AmPir,
animals were excluded if more than approximately 50% of mCherry+
cells were observed outside the AmPir.
Kondoh K., Lu Z., Ye X., Olson D.P., Lowell B.B, & Buck L.B. (2016). A specific area of olfactory cortex involved in stress hormone responses to predator odors. Nature, 532(7597), 103-106.
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