ARPE-19 cells were incubated for 48 h with normal (5 mM) and high (25 mM) glucose concentration solutions [17 (link)]. The cells were then collected, stained with trypan blue, and counted under an optical microscope. Cell counting kit-8 (CCK-8) detection kit (Dojindo Laboratories, Japan) was used to treat cells and the plate incubated at 37°C for 1 h following treatment with normal or and high glucose concentration solutions. Protein absorbance was measured at 450 nm using a monochromator microplate reader [18 (link)]. The OD values reflected the proliferation ability of the ARPE-19 cells.
Cck 8 detection kit
Manufactured by Dojindo Laboratories
Sourced in Japan, China
The CCK-8 detection kit is a colorimetric assay used to measure cell viability and proliferation. It utilizes the water-soluble tetrazolium salt WST-8, which produces a water-soluble formazan dye upon reduction by cellular dehydrogenases. The amount of formazan dye produced is directly proportional to the number of living cells, allowing for quantitative analysis of cell proliferation and cytotoxicity.
Automatically generated - may contain errors
Lab products found in correlation
Synergy h4 hybrid reader, by Agilent Technologies (2 mentions)
Spectramax 190, by Eppendorf (2 mentions)
Facsaria 2, by BD (2 mentions)
Microplate spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Multiskan spectrum, by Agilent Technologies (1 mentions)
Adriamycin, by Merck Group (1 mentions)
Pd173074, by Merck Group (1 mentions)
Paclitaxel, by Merck Group (1 mentions)
Mqx200, by Agilent Technologies (1 mentions)
Edu assay kit, by Thermo Fisher Scientific (1 mentions)
Fluorescent microscope, by Olympus (1 mentions)
Microplate reader, by Bio-Rad (1 mentions)
Pharmingen brdu flow kit, by BD (1 mentions)
Annexin 5 pe assay kit, by BD (1 mentions)
24 protocols using cck 8 detection kit
1
ARPE-19 Glucose Cytotoxicity Assay
2
Cell Viability Assay with CCK-8
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The viability of BV2 cells was determined using a CCK-8 detection kit (Dojindo, Kyushu, Japan) as previously described [28 (link)].
3
Cell Viability Assay of 5-FU on Cancer Cells
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Cell viability assay was performed using the CCK8 detection kit (Dojindo, Kumamoto, Japan) as described by the manufacturer. Briefly, cells were seeded in 96-well flat-bottomed microtitre plates at a density of 5000 cells/well. After 24 h, the medium was replaced by medium without or with indicated 5-fluorouracil (5-FU; 120 μg/ml for AGS; 200 μg/ml for SW620) and incubated for 24 h. Absorbance was measured on a microplate reader (Synergy H4 Hybrid Reader, Bio-Tek, Winooski, VT, USA) at a wavelength of 450 nm. The experiments were performed in triplicate.
4
CCK-8 Proliferation Assay Protocol
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CCK8 detection kit (Dojindo, Kumamoto, Japan) was applied to monitor the cell proliferation activity at 24, 48, and 72 h after inoculation according to the manufacturer’s protocol. In brief, experimental BLCA cell lines were inoculated into the 96-well plate at a density of 5000 cells/well. After 48 h of transfection, CCK8 reagent (10 μL) was supplemented into each well and cultured at room temperature. Then, DMSO (150 μL) was added and the absorbance at 450 nm was tested via a microplate spectrophotometer (Thermo Labsystems, Vantaa, Finland). CCK-8 assay was repeated 3 times.
5
Cell Proliferation Assay with Paclitaxel, Adriamycin, and PD173074
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To measure the proliferation of different transfected cloned cell lines, a CCK-8 detection kit (Dojindo Molecular Technologies, Inc.) was used, according to the manufacturer's protocol. In total, ~3,000 cells were seeded into a 96-well plate in quintuplicate for 6 h, and complete medium was then changed to DMEM with different concentrations (0, 10, 100, 1,000 and 10,000 nM) of paclitaxel, Adriamycin or PD173074 (Sigma-Aldrich; Merck KGaA) for 24 h or the cells were cultured without FBS for 0, 24, 48, 72, 96, 120 and 144 h at 37°C. Next, 10 µl CCK-8 reagent was added to each well, and the absorbance value was measured at 490 nm using a Multiskan Spectrum spectrophotometer (BioTek Instruments, Inc.).
6
Cell Viability Assay Using CCK-8
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Cell viability was determined utilizing CCK-8 detection kit (Dojindo, Shanghai, China). The cells in each group were seeded onto 96-well plates. Each well was treated with 10 μL CCK-8 solution at 37°C for 3-4 h. After adding 10 μL stop solution to each well, the OD450 value was determined with a microplate reader.
7
CCK-8 Assay for Cell Viability Assessment
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The cell viability was evaluated by the CCK-8 detection kit (Dojindo). 2BS or WI-38 cells were seeded into 96-well plates (5000/well), and then treated with drugs such as oridonin for 24 h. 1:10 diluted CCK-8 solution was added to the culture medium and incubated for 1 h at 37°C. Then a microplate reader (Biotek, MQX200) was used to measure the absorbance at 450 nm.
8
Cell Viability Assay with CCK8 Kit
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The cell viability assay was performed using the CCK8 Detection Kit (Dojindo, Kumamoto, Japan) according to the manufacturer’s instructions. Cells were seeded in a 96-well plate at a density of 4,000 cells/well. The absorbance was measured on a microplate reader (Synergy H4 Hybrid Reader; BioTek Instruments, Inc., Winooski, VT, USA) at a wavelength of 450 nm. The experiments were performed in triplicate.
9
Measuring Cell Viability with CCK-8
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Cell viability was assessed using a CCK-8 detection kit (Dojindo Molecular Technologies, Inc., Kumamoto, Japan), according to the manufacturer's protocol. Briefly, 1×105 cells/well were cultured in 96-well plates. Cells were treated with BAY11-7082 or MJ33, as described, and exposed to ischemic and hypoxic conditions for different periods of time. Subsequently, 100 µl 10% CCK-8 solution was added per well and then incubated at 37°C for 1 h. Absorbance was measured at 450 nm using a microplate reader (SpectraMax 190; Eppendorf, Hamburg, Germany).
10
Cell Viability Quantification Using CCK-8
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Counting kit-8 (CCK-8) detection kit (CK04; Dojindo, Shanghai, China) was utilized for detecting the cell viability. Primary astrocytes were seeded onto a 96-well plate (104 cells/well). Each group was set to three repetitions. After incubation overnight, 20 μl of CCK-8 was added to each well and cells were incubated lasting 4 h. The absorbance values at 450 nm were examined with a microplate reader.
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