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Fibroblast growth factor 4

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Fibroblast growth factor 4 is a protein that stimulates the growth and division of fibroblast cells. It is involved in the regulation of various cellular processes, including cell proliferation, differentiation, and migration.

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Lab products found in correlation

Heparin, by Merck Group (2 mentions) 2 mercaptoethanol, by Merck Group (2 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) D mannitol, by Merck Group (1 mentions) Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Corning (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Hepatocyte growth factor (hgf), by Thermo Fisher Scientific (1 mentions) Insulin transferrin selenium, by Merck Group (1 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions) Insulin transferrin selenium, by Thermo Fisher Scientific (1 mentions) Oncostatin m, by Thermo Fisher Scientific (1 mentions) Fibroblast growth factor 4, by Thermo Fisher Scientific (1 mentions) Activin a, by Novoprotein (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Nicotinamide, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by MP Biomedicals (1 mentions) Sodium pyruvate, by Corning (1 mentions)

4 protocols using fibroblast growth factor 4

1

Rat Trophoblast Stem Cells under Hyperglycemia

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Blastocyst-derived rat TS cells15 (link) were cultured in basal culture medium (RPMI 1640 (Cellgro), 20% fetal bovine serum (Sigma-Aldrich), 100 µM 2-mercaptoethanol (M7522; Sigma-Aldrich), 1 mM sodium pyruvate (ThermoFisher, 11 360–070), 50 µM penicillin (15140122; ThermoFisher), and 50 U/mL streptomycin (15140122; ThermoFisher)) supplemented with 70% rat embryonic fibroblast-conditioned medium, fibroblast growth factor 4 (25 ng/mL; Sigma-Aldrich), and heparin (1 µg/mL; Sigma-Aldrich). To model hyperglycemia, 25 mM glucose (Sigma-Aldrich) was added to the culture medium. D-mannitol (Sigma-Aldrich) was added to the culture medium to control for osmolality. Rat TS cells were exposed to ambient or low oxygen (0.5% or 1.5% O2) tensions and 5% CO2 at 37°C in an NAPCO 8000 incubator (ThermoFisher) for 24 hours and then harvested for immunocytochemical or biochemical analyses.
Nteeba J., Varberg K.M., Scott R.L., Simon M.E., Iqbal K, & Soares M.J. (2020). Poorly controlled diabetes mellitus alters placental structure, efficiency, and plasticity. BMJ Open Diabetes Research & Care, 8(1), e001243.
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2

Stepwise Hepatocyte Differentiation from UC-MSCs

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Hepatocytes were differentiated as described,53 (link) with some modifications. The differentiation process was divided into three stages: pretreatment step, differentiation step and maturation step. Passage 2-Passage 8 UC-MSCs were seeded at 4×103 cells/cm2 in six-well plates in serum-free medium. Culture medium was switched 24 h later to pretreatment medium based on the serum-free medium supplemented with 20 ng/ml epidermal growth factor (Peprotech), 100 ng/ml activin A (Novoprotein) and 10 ng/mL fibroblast growth factor 4 (Peprotech) for 3 days. Thereafter, differentiation was induced by treating UC-MSCs with serum-free medium containing 20 ng/ml hepatocyte growth factor (Peprotech), 10 ng/mL fibroblast growth factor 4, 0.61 g/L nicotinamide (Sigma), 1% dimethyl sulfoxide (MP Biomedicals), and 1% insulin-transferrin-selenium (Gibco) for 10 days. Then cells were incubated with maturation medium containing 20 ng/mL oncostatin M (Peprotech), 1 μmol/L dexamethasone (Sigma), 1% dimethyl sulfoxide and 1% insulin-transferrin-selenium for 15 days. Medium was changed every 3 days.
Jin M., Yi X., Zhu X., Hu W., Wang S., Chen Q., Yang W., Li Y., Li S., Peng Q., Pan M., Gao Y., Xu S., Zhang Y, & Zhou S. (2024). Schisandrin B promotes hepatic differentiation from human umbilical cord mesenchymal stem cells. iScience, 27(2), 108912.
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3

Transduction and Blastocyst Outgrowth Analysis

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Rat embryos were transduced with lentiviral particles as previously described (34 (link)–37 ). Blastocyst outgrowth analysis was performed as reported before (38 (link), 39 (link)). Briefly, blastocysts were treated with Acid Tyrode’s solution (Millipore, Darmstadt, Germany) to remove the zona pellucida from each blastocyst and incubated with concentrated lentiviral particles for 4.5 h. Transduced blastocysts were cultured ex vivo in TS Cell Complete Medium [RPMI 1640 (Cellgro, Herndon, VA), 20% FBS (Atlanta Biologicals, Norcross, GA), 100 μM 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO), 1 mM sodium pyruvate (Cellgro, Herndon, VA), 50 μM penicillin and 50 U/ml streptomycin (Cellgro)] supplemented with 70% rat embryonic fibroblast conditioned medium, fibroblast growth factor 4 (25 ng/ml; Sigma-Aldrich) and heparin (1 μg/ml; Sigma-Aldrich) for blastocyst outgrowth analysis. After 4 days the attached blastocysts were either fixed in 4% paraformaldehyde solution, or collected for RNA isolation (Picopure RNA Isolation Kit, Thermo Fisher, Waltham, MA). The surface area of blastocyst outgrowth was visualized using light microscopy and measured using Image J software.
Kubota K., Iqbal K, & Soares M.J. (2020). SATB1 PROMOTION OF TROPHOBLAST STEM CELL RENEWAL THROUGH REGULATION OF THREONINE DEHYDROGENASE. Biochimica et biophysica acta. General subjects, 1865(1), 129757.
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4

Monocyte Differentiation to NeoHep

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The differentiation of monocytes to NeoHep was achieved by using a two‐step differentiation protocol. In the first step, monocytes were differentiated to “reprogrammed monocytes” (RM) in the presence of basal Iscove's modified Dulbecco's medium (IMDM; Thermo Fisher Scientific, Waltham, MA,
https://www.thermofisher.com), supplemented with interleukin‐3, macrophage colony‐stimulating factor (Prospec, Ness‐Ziona, Israel,
http://www.prospecbio.com), β‐mercaptoethanol (Sigma, St. Louis, MO,
https://www.sigmaaldrich.com), and 0.5% embryonic stem cell‐grade fetal bovine serum (eFBS) (Biological Industries, Kibbutz Beit‐Haemek, Israel,
http://www.bioind.com). After day 6, RM were differentiated to NeoHep in 15 days in the presence of IMDM, supplemented with epithelial growth factor (Prospec), hepatocyte growth factor (HGF), fibroblast growth factor‐4, linoleic acid (Sigma), and eFBS. Detailed steps are given in the
supplemental online data.
Bhattacharjee J., Das B., Sharma D., Sahay P., Jain K., Mishra A., Iyer S., Nagpal P., Scaria V., Nagarajan P., Khanduri P., Mukhopadhyay A, & Upadhyay P. (2016). Autologous NeoHep Derived from Chronic Hepatitis B Virus Patients’ Blood Monocytes by Upregulation of c‐MET Signaling. Stem Cells Translational Medicine, 6(1), 174-186.
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