Spot digital camera

Manufactured by Nikon

Sourced in Japan

The Spot digital camera is a high-quality imaging device designed for scientific and industrial applications. It captures detailed digital images with precise color reproduction and high resolution. The camera's core function is to provide clear, accurate visual data for laboratory analysis and documentation purposes.

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Lab products found in correlation

Quantstudio 3 real time pcr system, by Thermo Fisher Scientific (2 mentions) Euparal mounting medium, by Carl Roth (1 mentions) E1000 microscope, by Nikon (1 mentions) Splscar block, by SPL Life Sciences (1 mentions) Biotinylated antibodies, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Phagocytosis assay kit, by Cayman Chemical (1 mentions) Cd11b magnetic separation kit, by STEMCELL (1 mentions) Luminex multiplex cytokine assay, by Thermo Fisher Scientific (1 mentions) Odyssey gel imager, by LI COR (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions) Celltiter glo luminescent cell viability assay kit, by Promega (1 mentions) Cell culture supplies, by Corning (1 mentions) Slide scanner, by 3DHISTECH (1 mentions) Histochoice clearing agent, by Merck Group (1 mentions)

Close spelling variants of this product

Spot RT digital camera SPOT RTTM digital camera Spot RT Slider digital camera Digital camera

4 protocols using spot digital camera

Wing Preparation for Microscopy

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Wings were removed from adult flies, dehydrated in 100% ethanol for 5 min and placed on a microscope slide to allow ethanol to evaporate. A small drop of Euparal Mounting Medium (Roth, Karlsruhe, Germany) was dropped onto the wing and a glass coverslip placed on top. Images were captured with a Spot digital camera and a Nikon E1000 microscope (Nikon Instruments Europe, Tokyo, Japan).

Vicidomini R., Di Giovanni A., Petrizzo A., Iannucci L.F., Benvenuto G., Nagel A.C., Preiss A, & Furia M. (2015). Loss of Drosophila pseudouridine synthase triggers apoptosis-induced proliferation and promotes cell-nonautonomous EMT. Cell Death & Disease, 6(3), e1705-.

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Cell Migration Assay Using SPLScar™ Block

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The 12-well plate with SPLScar™ Block (SPL Life Sciences Co. Ltd., Pocheon-si, Korea) in each well was used for migration assay. 5 X 10⁴ cells suspended in normal culture medium were seeded in the block. After 24 h, the wall (500 μM thick) was removed by sterile forceps to artificially generate a 500 μm of cell-free gap. The cell gap was monitored by a light microscope (Nikon, Japan) equipped with Spot digital camera and the gap distance was measured at 0, 16, and 24 h after the wall was removed. The migratory distance was defined as 500 μm minus the cell-free gap distance.

Liu C.C., Yu C.F., Wang S.C., Li H.Y., Lin C.M., Wang H.H., Abate C, & Chiang C.S. (2019). Sigma-2 receptor/TMEM97 agonist PB221 as an alternative drug for brain tumor. BMC Cancer, 19, 473.

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Multimodal Characterization of Macrophage Response

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Dulbecco’s modified Eagle’s medium (DMEM), DMEM-F12, fetal bovine serum (FBS), L-glutamine (Q), penicillin/streptomycin (P/S), Sodium Pyruvate (SP), and Non-Essential Amino Acids (NEAA) were obtained from Invitrogen (Carlsbad, CA). CellTiter Glo Luminescent Cell Viability Assay kit was obtained from Promega (Madison, WI). The CD11b magnetic separation kit was purchased from Stem Cell Technologies (Vancouver, Canada). All standards used for the Luminex multiplex cytokine assay were purchased from PeproTech, Inc (Rocky Hill, NJ). Streptavidin-Biotin and biotinylated antibodies used for Luminex were purchased from eBioSciences (San Diego, CA). Phagocytosis assay kit was purchased from Cayman Chemicals (Ann Arbor, MI).
For qRT-PCR, the QuantStudio3 Real Time PCR system from Thermo Fisher (Waltham, MA) was used. For MTS and Griess assays, a Molecular Devices (Sunnyvale, CA) plate reader was used. For scanning Western blot, a Li-COR Odyssey gel imager (Lincoln, NE) was used. Cell culture supplies were purchased from Corning (Corning, NY). For immunocytochemistry studies, the images were obtained using a Nikon (Model: TE-2000U) inverted fluorescent microscope and images were obtained using a Spot Digital Camera (Sterling Heights, MI).

Sarkar S., Malovic E., Sarda D., Lawana V., Rokad D., Jin H., Anantharam V., Kanthasamy A, & Kanthasamy A.G. (2018). Characterization and comparative analysis of a new mouse microglial cell model for studying neuroinflammatory mechanisms during neurotoxic insults. Neurotoxicology, 67, 129-140.

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Histological Examination of Liver Tissues Post-DEN Injection

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After taking the PET-CT scan at minus 2, 0, plus 2, and plus 4 weeks after the last DEN injection, animals were sacrificed for histological examination. The rats (n=5, for each week) were deeply anesthetized with 15% urethane and sacrificed by decapitation. Then the liver was immediately removed and fixed in 10% formalin for 24 hr. The liver tissues were embedded in a paraffin block and cut into slices with a thickness of 4 µm for hematoxylin and eosin (H&E) staining. The sections were dewaxed in a histochoice clearing agent (Sigma) and rehydrated through a graded alcohol series. The slides were stained with H&E, and images were acquired using a slide scanner (3D Histech, Budapest, Hungary) and a microscope equipped with a spot digital camera (Nikon, Chiyoda-ku, Tokyo, Japan) (× 200).

Park S.I., Lee J.H., Ham H.J., Jung Y.J., Park M.S., Lee J., Maeng L.S., Chung Y.A, & Jang K.S. (2015). Evaluation of 2-[18F]-fluoro-2-deoxy-D-glucose positron emission tomography/computed tomography in rat models with hepatocellular carcinoma with liver cirrhosis. Bio-medical materials and engineering, 26 Suppl 1.

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