ACR, ethylene glycol tetra-acetic acid (EGTA), atropine, mecamylamine hydrochloride, Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME), nereistoxin (NTX), and phenylephrine (PE) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Sodium chloride, KCl, calcium chloride dehydrate, potassium dihydrogen phosphate, magnesium sulphate heptahydrate, sodium hydrogen carbonate, and glucose were purchased from Merck (Darmstadt, Germany).
Calcium chloride dehydrate
Manufactured by Merck Group
Sourced in United States, Germany, United Kingdom, Spain
Calcium chloride dehydrate is a chemical compound with the formula CaCl2·2H2O. It is a white crystalline solid that is highly soluble in water. The primary function of calcium chloride dehydrate is to act as a desiccant, absorbing moisture from the surrounding environment.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Merck Group (16 mentions)
Potassium chloride (kcl), by Merck Group (7 mentions)
Magnesium chloride hexahydrate, by Merck Group (6 mentions)
Sodium bicarbonate, by Merck Group (6 mentions)
D glucose, by Merck Group (5 mentions)
Potassium dihydrogen phosphate, by Merck Group (4 mentions)
Hydrochloric acid (hcl), by Merck Group (4 mentions)
Bovine serum albumin (bsa), by Merck Group (4 mentions)
Citric acid, by Merck Group (4 mentions)
Triton x 100, by Merck Group (3 mentions)
Magnesium sulfate heptahydrate, by Merck Group (3 mentions)
Anhydrous sodium carbonate, by Merck Group (3 mentions)
Tannic acid, by Merck Group (3 mentions)
Boric acid, by Merck Group (3 mentions)
Sodium hydrogen carbonate, by Merck Group (2 mentions)
Nω nitro l arginine methyl ester hydrochloride, by Merck Group (2 mentions)
Sodium hydroxide pellet, by Merck Group (2 mentions)
Ethylene glycol tetraacetic acid (egta), by Merck Group (2 mentions)
L glutamic acid, by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
39 protocols using calcium chloride dehydrate
1
Cardiovascular Pharmacology Reagents
2
Optimized Cell Culture Reagents Protocol
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Albumin Bovine Fraction V, pH 7.0, was from Serva (Huissen, The Netherlands), 2-butanol, glutardialdehyde solution 25%, magnesium chloride hexahydrate, paraformaldehyde (PFA), sodium hydroxide pellets, Triton X-100 were from Merck (Amsterdam, The Netherlands), calcium chloride dehydrate, catalase from Aspergillus niger, Dulbecco's modified Eagle's medium, EGTA, gelatin from porcine skin (300 g Bloom), glucose oxidase ethanol, hydrochloric acid, MES hydrate, phosphate buffered saline (PBS) tablets, petroleum ether, sodium borohydride were from Sigma-Aldrich (Zwijndrecht, The Netherlands). Cysteamine hydrochloride–MEA, D-(+)-glucose anhydrous were from Fluka (Zwijndrecht, The Netherlands). Murine epidermal growth factor (EGF) was from Invitrogen (Breda, The Netherlands). Fetal bovine serum (FBS) was from APS (Bedford, UK). PIPES was from Fisher Scientific (Breda, The Netherlands). Precision tissue wipes were from Kimtech Science (Ede, The Netherlands).
3
Compound Characterization and Stability of JME-173
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Sodium chloride, potassium chloride, potassium dihydrogen phosphate, sodium hydrogen carbonate, magnesium sulfate heptahydrate, and calcium chloride dehydrate were purchased from Merck (Darmstadt, Germany). Glucose, EGTA, carbachol, methacholine, theophylline, mexiletine, propranolol, SQ22,536, lipopolysaccharide (LPS), sodium pentobarbital, pancuronium bromide, sodium orthovanadate, and tetraethylammonium (TEA) were purchased from Sigma-Aldrich (St. Louis, MO). Isoflurane was obtained from Cristália (São Paulo, Brazil). JME-173 was synthesized and provided by the Laboratory of Organic Chemistry (Farmanguinhos, FIOCRUZ, RJ, Brazil). GCMS spectrum revealed that JME-173 is a 100% pure compound as also attested by the 1H NMR analysis and by its melting point 220–222°C. As predicted by the ChemAxon program (Version 19.22.0), the aqueous solubility at pH 6.4, 6.8, and 7.2 of JME-173 were 127.0, 50.6, and 20.2 mg/ml, respectively. There was no observed evidence of JME-173 degradation following long-lasting exposure to blood samples, storage at room temperature or −70°C, or after the repeated freeze and thaw cycles, pointing out the excellent physical stability of this compound (Pinto at al., under review in the Eur. J. Pharmacol.). All solutions were freshly prepared in distilled water and protected from light.
4
Comprehensive Cellular Analysis Protocol
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Reagents used in experiments were purchased from Sigma-Aldrich (St. Louis, Mo): sodium chloride, potassium chloride, D-glucose, N-2-hydroxyethylpiperazineN′-2-ethanesulfonic acid (HEPES), soybean trypsin inhibitor, bovine serum albumin (BSA), sodium pyruvate, L-glutamine, L-glutamic acid, L-alanine, Llysine, L-arginine, L-asparagine, L-aspartic acid, L-histidine, FCCP, collagenase type IV, proteinase K, Hoechst 33358, ethidium bromide, ethylenediaminetetraacetic acid (EDTA), agarose, nonyl phenoxypolyethoxylethanol (NP-40), Triton X-100, tris(hydroxymethyl)aminomethane (Tris). Calcium chloride dehydrate was purchased from Merck Chemicals (Burlington, Mass). All other reagents were of the purest available grade.
5
Colistin Resistance Profiling Protocol
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Gradient test MIC determination (Etest, bioM erieux, Marcy l'Etoile, France) was performed according to the manufacturer's instructions. Colistin disc diffusion was performed with 10-, 25and 50-mg discs (Oxoid/Thermo Fisher Scientific, Basingstoke, UK) adhering to EUCAST disc diffusion methodology [5] . MH agar plates were prepared in house according to the manufacturer's instructions using BBL (BD, Sparks MD, USA) or Oxoid (Thermo Fisher Scientific, Basingstoke, UK) MH agar powder without and with addition of calcium chloride dehydrate (Merck, Darmstadt, Germany) before autoclaving as described by Gwozdzinski et al. [4] . Testing was performed in four phases, see Table 1. Colistin MIC values, the occurrence of colistin resistance genes, and information of in which phase of the study each isolate was included are presented in Table S1.
6
Fabrication of Liquid Crystalline Surfaces
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Glass slides and Decon-90 were purchased from Fisher scientific (Waltham, MA, USA). BSA, N,N-Dimethyl-N-octadecyl-3-Aminopropyltrimethoxyysilychloride (DMOAP), Ammonium Chloride (NH4Cl), Hydrogen Chloride (HCl) were purchased from Sigma Aldrich (Singapore), 4–cyano-4-n-pentylbiphenyl (5 CB) was purchased from Merck (Singapore), while calcium chloride dehydrate (CaCl2), sodium Chloride (NaCl), Copper (I) Bromide(CuBr2), Copper (II) Sulfate (CuSO4), Copper (II) Nitrate [Cu(NO3)2], Copper (II) Perchlorate [Cu(ClO4)2.6H2O], Mercury (II) Chloride (HgCl2), Cobalt (II) Sulphate Co(SO4) and Lead (II) Chloride (PbCl2) were purchased from Alfa Asar (Singapore).
7
Analytical Standards Characterization Protocol
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Standards of sarcosine 98 %, α-alanine ≥ 99 % and β-alanine (BioUltra) ≥ 99.0 % (NT), glycine ≥ 99 % (HPLC), creatinine anhydrous > 98 % purity and ammonium chloride 99.5 %, A.C.S. were purchased from Sigma-Aldrich Corporation (Sigma-Aldrich, Saint Louis, MO, USA). N, O-Bis-(trimethylsilyl) trifluoroacetamide with 1 % trimethylchlorosilane [BATFA + TMCS, 99:1 (Sylon BFT)], derivatization grade for GC, was obtained from SUPELCO (Bellefonte, USA). Acetonitrile and water for chromatography grade were procured from Merck (Darmstadt, Germany). Urea 99.5 % AR and potassium phosphate dibasic AR were purchased from Loba Chemie Pvt. Ltd., (Mombai, India). Potassium chloride, calcium chloride dehydrate, sodium sulfate anhydrous, and sodium chloride were purchased from Merck.
8
Pyrazine-based Odor Compound Screening
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Dulbecco´s MEM (#F0435), FBS superior (#S0615), Lglutamine (#K0282), penicillin (10 000 U/mL)/streptomycin (10 000 U/mL) (#A2212), trypsin/EDTA solution (#L2143) (formerly Biochrom, Berlin, Germany, now Merck KGaA), calcium chloride dehydrate (#22322.295), D-glucose (#101174Y), dimethyl sulfoxide (DMSO) (#83673.230), HEPES (#441476L), potassium chloride (#26764.230), sodium hydroxide (#28244.295) (VWR International GmbH, Darmstadt, Germany), sodium chloride (#1064041000, Merck KGaA, Darmstadt, Germany), and D-luciferin (beetle) monosodium salt (#E464X, Promega, Madison, USA), 2,5-dihydro-2,4,5-trimethylthiazoline (CAS# 4145-93-1) (#AB494350, abcr GmbH, Karlsruhe, Germany).
Pyrazines and other odorants are listed in Table S1. Compounds of particular interest for our experiments are listed alongside their assigned compound numbers, CAS numbers, structures, acronyms, and designation as KFO and/ or semiochemical in Table 1. Compounds utilized in the KFO screen were as previously published 38, 39 (Table S2).
Pyrazines and other odorants are listed in Table S1. Compounds of particular interest for our experiments are listed alongside their assigned compound numbers, CAS numbers, structures, acronyms, and designation as KFO and/ or semiochemical in Table 1. Compounds utilized in the KFO screen were as previously published 38, 39 (Table S2).
9
Bovine Lactoferrin Purification and Labeling
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The bioactive Lf from bovine whey was kindly supplied by the Tatua Cooperative Dairy Company Ltd (New Zealand). Poly-L-Lysine hydrochloride (PLL, Mw 15,000–30,000), BSA lyophilized powder (≥96%), TA (ACS grade), BSA conjugated with fluorescein isothiocyanate (BSA/FITC), bile salts (for microbiology), pancreatin from porcine pancreas (≥100 USP U/mg), pepsin from porcine gastric mucosa (3802 U/mg), mini protease inhibitor cocktail cOmplete™, hydrochloric acid, calcium chloride dehydrate, sodium bicarbonate, sodium chloride, sodium hydroxide, acetonitrile (for HPLC, ≥99.9%) were purchased from Sigma-Aldrich. Anhydrous sodium carbonate was purchased from Alfa Aesar. 0.1% aqueous solution of trifluoroacetic acid (TFA, LC/MS grade, ≥99.99%) was purchased from VWR Chemicals. DQ™ Red BSA was purchased from Molecular Probes Inc, USA. The bovine lactoferrin ELISA kit was purchased from Bethyl Laboratories, Inc., USA. Cyanine 7 N-hydroxysuccinimide ester (Cy7-NHS) was purchased from Lumiprobe Corporation, USA. Cy7-labelled Lf (Cy7-Lf) has been prepared according to a standard protocol provided by Lumiprobe Co and purified by dialysis. All chemicals were used as received without further purification. Deionized (DI) water (specific resistivity higher than 18.2 MΩcm) from Milli-Q plus 185 (Millipore) water purification system was used to make all solutions.
10
Monocrotaline-Induced Cardiac Injury Model
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