Cleaved caspase antibody sampler kit

Tivantinib, JNJ-38877605 and PHA-665752 were purchased from Selleck Chemicals (Houston, TX, USA) and prepared as 20 mM stock solutions in DMSO (Sigma-Aldrich, St. Louis, MO, USA). For in vivo experiments, Tivantinib and JNJ-38877605 were dissolved as previous described and administered via oral gavage at a dose of 10 ml/kg [21 (link), 27 (link)]. Paclitaxel and vincristine were purchased from Sigma-Aldrich (St. Louis, MO). Primary antibodies against MET, phospho-MET (Tyr1234/1235), AKT, phospho-Akt (Ser473), ERK1/2, phospho-ERK1/2 (Thr202/Tyr204), α-tubulin, Cdc25C, Cylin B1, p21, FasL, Fas and horseradish peroxidase (HRP)-conjugated secondary antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA). The antibody against Ki-67 was purchased from Dako (Santa Barbara, CA, USA). The cleaved caspase antibody sampler kit and GSK3 antibody sampler kit were purchased from Cell Signaling Technology (Beverly, MA). The antibody against GAPDH was a product of Kangchen Biotech (Shanghai, China). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies Inc. (Kumamoto, Japan).

Puromycin, polybrene, fetal bovine serum (FBS), medium, antibiotics and other cell culture reagents were purchased from Sigma (St. Louis, Mo). Cell counting kit -8 (CCK-8) was provided by Dojindo (Kumamoto, Japan). The fluorescence dyes, including EdU (5-Ethynyl-2′-deoxyuridine), DAPI (4′,6-diamidino-2-phenylindole), TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) and JC-1, were provided by Thermo-Fisher Invitrogen (Shanghai, China). The anti-POLRMT antibody was purchased from Abcam (ab228576, Shanghai, China). The cleaved caspase antibody sampler Kit (#9929) and anti-β-Tubulin (#2146) were provided by Cell Signaling Technologies (Beverly, MA).

The cleaved caspase antibody sampler kit (#9929), IGF2BP1, (#8482) and β-Tubulin (#2146) were purchased from Cell Signaling Technologies (Beverly, MA). Cell culture reagents were obtained from Hyclone Co. (Logan, UT). Puromycin, polybrene and other chemicals were purchased from Sigma-Aldrich (St. Louis, Mo).

Antibodies for p70 S6 Kinase (S6K1, Thr389, #9205), S6K1 (#9202), NSD3 (WHSC1L1, full length, #92056), Cleaved Caspase Antibody Sampler Kit (#9929), and β-Tubulin (#2146) were purchased from Cell Signaling Technologies (Beverly, MA, USA). All sequences, primers, and viral constructs were produced by Shanghai Genechem Co. (Shanghai, China). Cell culturing reagents, including medium, FBS (fetal bovine serum) and antibiotics, were obtained from Hyclone (Shanghai, China). Cell Counting Kit -8 (CCK-8) was obtained from Dojindo (Kumamoto, Japan). Fluorescence dyes, including EdU (5-ethynyl-2′-deoxyuridine), TUNEL (terminal deoxynucleotidyl transferase dUTP nick-end labeling), and JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine) were provided by Thermo-Fisher Invitrogen (Shanghai, China).