Tnf α 210 ta 020
TNF-α (210-TA-020) is a recombinant human tumor necrosis factor alpha (TNF-α) protein. TNF-α is a multifunctional proinflammatory cytokine produced by a variety of cells, including macrophages, lymphocytes, and endothelial cells. It plays a central role in inflammation and immune system regulation.
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5 protocols using tnf α 210 ta 020
AMPK Signaling in RPE Cells
Investigating Vaspin's Modulation of Inflammatory Responses in Human Aortic Endothelial and Monocytic Cells
THP-1 cells, a human monocytic cell line (ATCC® TIB-202™, Rockville, MD, USA), were grown in RPMI-1640 medium containing 10% fetal bovine serum. In all experiments, cells were used at six or fewer passages.
TNFα (210-TA-020, R&D Systems, Inc. Minneapolis, MN, USA) was used as the representative proinflammatory cytokine and phosphate buffered saline was used as the vehicle. Cells were transferred to medium containing 1% fetal bovine serum and incubated in media containing 10 ng/mL TNFα for the indicated amount of time. Vaspin was obtained from Adipogen Inc. (AG-40A0064, Incheon, Korea) and phosphate buffered saline was used as the vehicle. Cells were transferred to medium containing 1% fetal bovine serum and incubated in media containing various concentrations of Vaspin for the indicated amount of time before treatment with TNFα. 5-Aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (123040, Calbiochem, Darmstadt, Germany), an activator of AMPK, was used as a positive control.
TNF-α and CHX-Induced Cell Death
Cell Culture and Cytokine Treatments
Activation of S1P Signaling Pathways
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