Arachidonoyl 1 thio glycerol

Parathion (O,O′-diethyl-p-nitrophenyl-phosphorothioate) and chlorpyrifos (O,O′-diethyl-3,5,6-trichloro-2-pyridinyl-phosphorothioate), >99% pure; GC/MS analysis, were purchased from Chem Service (West Chester, PA). Acetylcholine iodide (acetyl-³H, specific activity 76.0 Ci/mmol) was purchased from Perkin Elmer (Wellesley, MA). [³H]anandamide (ethanolamine 1-³H) specific activity 60 Ci/mmol), was purchased from American Radiolabeled Chemicals (St. Louis, MO). Arachidonoyl-1-thio-glycerol and anandamide were purchased from Cayman Chemical (Ann Arbor, MI). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO). Guide cannulae (MD 2250) and microdialysis probes (MD 2204, 4 mm membrane) were purchased from Bioanalytical Systems Inc. (BAS, West Lafayette, IN).

Paraoxon (PO) and chlorpyrifos oxon (CPO were purchased from ChemService (West Chester, PA; both >98% purity). [³H]anandamide (ethanolamine-1-³H), specific activity 60 Ci/mmol) was purchased from American Radiochemical Company (St. Louis, MO). Arachidonoyl-1-thio-glycerol, anandamide, and AM 251 (N-(piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide) were purchased from Cayman Chemical (Ann Arbor, MI). Acetylthiocholine iodide and all other chemicals were purchased from Sigma-Aldrich (St. Louis, MO).