Rat anti mouse ly6g antibody

Manufactured by BioLegend

The Rat anti-mouse Ly6G antibody is a laboratory tool used for the identification and isolation of mouse Ly6G-positive cells, which are primarily neutrophils. This antibody specifically binds to the Ly6G antigen, allowing researchers to detect and study the behavior and function of these cells in various biological applications.

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Lab products found in correlation

Ctr6000, by Leica (1 mentions)

Spelling variants of this product

Anti-Ly6G (76 citations) Anti-Ly6G antibody (18 citations) Anti-mouse Ly6G (14 citations) Rat anti-mouse Ly6G (8 citations) Ly6G antibody (8 citations) Rat anti-Ly6G (7 citations) Anti-mouse Ly6G antibody (5 citations)

3 protocols using rat anti mouse ly6g antibody

Neutrophil Depletion in Atherosclerosis

Cited 1 time

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For neutrophil depletion experiments, Mep1α^−/− Apoe^−/− mice on a B6 genetic background, which show increased neutrophil infiltration in atherosclerotic lesions (unpublished data), were intraperitoneally (I.P.) injected with 200 μg of monoclonal rat anti-mouse Ly6G antibody (Clone: 1A8, Biolegend) per mouse every other day for 4 weeks, starting the day before surgery. For mice that were not treated with the antibody, an equivalent amount of saline was administered as reported [23 (link)].

Zhao J., Huangfu C., Chang Z., Grainger A.T., Liu Z, & Shi W. (2019). Atherogenesis in the carotid artery with and without interrupted blood flow of two hyperlipidemic mouse strains. Journal of vascular research, 56(5), 241-254.

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Neutrophil Depletion for Allergy Experiments

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To deplete neutrophils, adult hFcεRI/hNC16A mice were pretreated with i.p. injection of rat anti-mouse Ly6G antibody or match control antibody (BioLegend, cat#127601) at a dose of 150μg per mouse and 12 h later injected at ear with anti-hNC16A IgE or control IgE (Liu et al., 2008 (link)). Neutrophil levels in circulation were monitored by direct cell counting of blood smears stained with Wright dye (Baxter Diagnostics Inc., McGaw Park, IL).

Lin L., Hwang B.J., Culton D.A., Li N., Burette S., Koller B.H., Messingham K.A., Fairley J.A., Lee J.J., Hall R.P., An L., Diaz L.A, & Liu Z. (2017). Eosinophils mediate tissue injury in autoimmune skin disease bullous pemphigoid. The Journal of investigative dermatology, 138(5), 1032-1043.

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Immunohistochemical Detection of Ly-6G

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Paraformaldehyde-fixed, paraffin-embedded samples were sectioned at a thickness of 5 μm. Rat anti-mouse Ly-6G antibody (BioLegend) was used for immunohistochemistry according to established procedures50 (link). The areas in all specimens that stained positive for Ly-6G were examined using a microscope (CTR 6000; Leica, Wetzlar, Germany).

Wu D., Zeng Y., Fan Y., Wu J., Mulatibieke T., Ni J., Yu G., Wan R., Wang X, & Hu G. (2016). Reverse-migrated neutrophils regulated by JAM-C are involved in acute pancreatitis-associated lung injury. Scientific Reports, 6, 20545.

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