Anti α actin

Manufactured by Proteintech

Sourced in United States, United Kingdom

Anti-α-Actin is a primary antibody that binds specifically to the alpha-actin protein, a crucial component of the cytoskeleton in various cell types. This antibody can be used in techniques such as immunohistochemistry, immunocytochemistry, and Western blotting to detect and quantify the presence of alpha-actin in biological samples.

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Lab products found in correlation

2 protocols using anti α actin

Protein Expression Analysis of DJ-1, Apoptosis, and Cell Cycle Regulators

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Cells and clinical samples were lysed in RIPA buffer, and quantified by Rapid Gold BCA (Thermo, USA). Protein samples were separated equally by SDS-PAGE and electrotransferred to polyvinylidene difluoride (PVDF) membranes, which were then blocked for 1 h, and incubated with anti-DJ-1 (ab76008, Abcam, USA), anti-Bax (ab3191, Abcam, USA), anti-Bcl2 (ab196495, Abcam, USA), Anti-Cleaved Caspase-3(ab214430, Abcam, USA), Anti-CDK4(ab95255, Abcam, USA), Anti-Cyclin D1 (ab226977, Abcam, USA), Anti-E2F1 (ab137415, Abcam, USA), Anti-Cyclin D2 (ab230883, Abcam, USA), Anti-Cyclin D3 (ab112034, Abcam, USA), Anti-RB(17218-1-AP, proteintech, USA), Anti-pRb (phospho S780, ab47763,Abcam, USA), Anti-E2F1(12171-1-AP, proteintech, USA), Anti-H2A(16441-1-AP, proteintech, USA), Anti-α-Tubulin(11224-1-AP, proteintech, USA), Anti-Flag(80010-1-RR, proteintech, USA), and Anti-α-Actin(23660-1-AP, proteintech, USA). Next, the membranes were incubated in corresponding secondary antibodies for 1 h. Proteins were visualized and detected by SuperSignal West Atto(A38554, Thermo, USA).

Han Z., Wang L., Wang D., Zhang L., Bi Y., Zheng X., Liu W., Bai G., Wang Z., Wan W., Ma Y., Cai X., Liu T, & Jia Q. (2022). DJ-1 promotes osteosarcoma progression through activating CDK4/RB/E2F1 signaling pathway. Frontiers in Oncology, 12, 1036401.

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Detailed Antibody Characterization Protocol

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The following primary antibodies were used: anti‐p53 (Abcam, UK), anti‐p16^INK4a (Abcam, UK), anti‐p16^INK4a (Abcam, UK), anti‐p21^CIP1/WAF1 (Abcam, UK), anti‐GATA4 (Santa‐Cruz, USA), anti‐GATA4 (Abcam, UK), anti‐CCN1 (Abcam, UK), anti‐γ‐H2AX (Abcam, UK), anti‐γ‐H2AX (CST, USA), anti‐GAPDH (Proteintech, China), anti‐β‐actin (Boster, China), anti‐interleukin (IL)‐1α (Proteintech, China), anti–monocyte chemotactic protein‐1 (Abcam, UK), anti–tumor necrosis factor‐α (TNF‐α) (Abcam, UK), and anti‐α‐actin (Proteintech, China). All secondary antibodies were from ZSGB‐BIO Company (China). Reagents wheat germ agglutinin and Alda‐1 were purchased from Sigma‐Aldrich, USA. All of the catalog numbers of the antibodies are listed in Table S1.

Cui S., Xue L., Yang F., Dai S., Han Z., Liu K., Liu B., Yuan Q., Cui Z., Zhang Y., Xu F, & Chen Y. (2018). Postinfarction Hearts Are Protected by Premature Senescent Cardiomyocytes Via GATA4‐Dependent CCN1 Secretion. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 7(18), e009111.

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