Pfd 425s peltier thermostated cell holder

The measurements were performed by adding aliquots of DMSO stock solution of the tested compounds to the buffer solution (pH 7.0, 20 mM, cacodylate buffer, DMSO content of the final solutions = 0.22–1.30%). Changes in the CD spectrum of ct-DNA upon the addition of compound were measured at different molar ratios r = [compound]/[ct-DNA]. CD spectra were recorded on a JASCO J-815 CD spectrometer with a JASCO PFD-425S Peltier thermostated cell holder (JASCO, Tokyo, Japan) by using a square quartz cuvette of path length 0.5 cm (0.5 ml) in the 230–400 nm region. The CD profiles reported were an average of three successive scans with a scan time of 200 nm per minute and an appropriately corrected baseline. Temperature was maintained at 20 °C during the experiment.

The measurements were performed by adding aliquots of DMSO stock solution of the tested compounds to the buffer solution (pH 7.0, 20 mM, cacodylate buffer, DMSO content of the final solutions = 0.15–1.22%). Changes in the CD spectrum of ct-DNA upon the addition of compound were measured at different molar ratios r = [compound]/[ct-DNA]. Circular dichroism spectra were recorded on a JASCO J-815 CD spectrometer with JASCO PFD-425S Peltier thermostated cell holder (Tokyo, Japan) using a rectangular quartz cuvette of path length 0.5 cm (1 mL) in the 230–400 nm region. The reported CD profiles are an average of three successive scans with 200 nm per minute scan time and an appropriately corrected baseline. The temperature was maintained at 20 °C during the experiment.