Iii chloride hexahydrate

Where indicated, cells were treated with gadolinium (III) chloride hexahydrate (25 μM, Sigma-Aldrich), BAPTA-AM (10 μM, Abcam) or JIB4 (10 μM, Tocris), which were added to the culture 15 min prior to mechanical stretch/compression. Thapsigargin (2 μM, Abcam) was added at the start of live imaging of stretch. The vehicle dimethyl sulfoxide (DMSO; Sigma-Aldrich) was used as a control for all treatments.

Acetone
(ACS reagent grade,
≥99.5%), ethanol (EtOH) (analytical standard for GC, ≥99.9%),
ammonium hydroxide solution (NH₃·H₂O) (ACS
reagent grade, 28.0–30.0 wt % NH₃ basis), magnesium
nitrate hexahydrate [Mg(NO₃)₂·6H₂O] (ACS reagent grade, ≥99.0%), zinc chloride hexahydrate
[ZnCl₂·6H₂O] (ACS reagent grade, ≥99.0%),
zinc nitrate hexahydrate [Zn(NO₃)₂·6H₂O] (ACS reagent grade, ≥99.0%), aluminum nitrate nonahydrate
[Al(NO₃)₃·9H₂O] (ACS reagent
grade, ≥99.0%), aluminum chloride hydrate [AlCl₃·xH₂O] (purity 99.999%), iron(III)
chloride hexahydrate [FeCl₃·6H₂O] (ACS
reagent grade, ≥97.0%), and sodium hydroxide [NaOH] (ACS reagent
grade, ≥97.0%) were purchased from Sigma-Aldrich (Milano, Italy).
The common household bleach based on sodium hypochlorite (NaClO) (solution
with 2.2 wt %) was employed. All of the chemicals were used as received
without further purification. Si 4″ wafers finished with a
1 μm thick thermally grown SiO₂ layer were purchased
from Si-Mat (Kaufering, Germany) and used as substrates for device
fabrication.