Sk ov3

Human epithelial ovarian cancer cell lines (SK-OV3, ES-2, A2780) and HEK293T cell line were purchased from the American Type Culture Collection (VA, USA). SK-OV3 and ES-2 cells were cultured in McCoy's 5A medium (#X054B1, BasalMedia) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS, #10270-106, Gibco), 100 unit/mL penicillin and 100 µg/mL streptomycin (P/S, #SV30010, Hyclone), while A2780 and HEK293T cells were grown in DMEM (#21041025, Gibco) supplemented with 10% (v/v) FBS and P/S. Opti-MEM (#31985) were acquired from Gibco (Waltham MA USA). Cells were cultured in a humidified environment of 95% air, 5% CO₂ at 37°C. Experiments were performed when the cells reached 90% confluence.

Human ovarian cancer cell lines CAOV3, OVCAR3, SKOV3, and UWB1.289 were purchased from the American Type Cell Culture. A2780 was purchased from Sigma (Cat# 93112519). HO8910 was purchased from National Collection of Authenticated Cell Cultures of China (Cat# TCHu 24). SKOV3 cells (Cat# HTB-77) were cultured in McCoy’s 5A (BasalMedia, L630KJ). UWB1.289 cells (Cat# CRL-2945) were grown in RPMI 1640 (Cellmax, CGM112.05) and MEGM Bullet Kit (Lonza, CC-3150) at a 1:1 ratio. CAOV3 (Cat# HTB-75) and OVCAR3 (Cat# HTB-161) were cultured in Dulbecco’s Modified Eagle’s Medium (BasalMedia, L110KJ). A2780 and HO8910 were grown in RPMI 1640 (BasalMedia, L210KJ). All culture media contained 10% fetal bovine serum (Gibco, 7471) and 1% penicillin/streptomycin (Invitrogen, 15140-122). The cells were determined to be mycoplasma-free and cultured at 37 °C with 5% CO₂.